LOCUS       sequence-168329-        4650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ELF1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ELF1_p.P32S
FEATURES             Location/Qualifiers
     source          1..4650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2529..2628)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2645..2664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2646..2664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2669..2685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2669..2685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3116..3907
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4000..4588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4489..4508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggctgctg ttgtccaaca gaacgaccta gtatttgaat ttgctagtaa
      721 cgtcatggag gatgaacgac agcttggtga tccagctatt ttttctgccg taattgtgga
      781 acatgttcct ggtgctgata ttctcaatag ttatgccggt ctagcctgtg tggaagagcc
      841 caatgacatg attactgaga gttcactgga tgttgctgaa gaagaaatca tagacgatga
      901 tgatgatgac atcaccctta cagttgaagc ttcttgtcat gacggggatg aaacaattga
      961 aactattgag gctgctgagg cactcctcaa tatggattcc cctggcccta tgctggatga
     1021 aaaacgaata aataataata tatttagttc acctgaagat gacatggttg ttgccccagt
     1081 cacccatgtg tccgtcacat tagatgggat tcctgaagtg atggaaacac agcaggtgca
     1141 agaaaaatat gcagactcac cgggagcctc atcaccagaa cagcctaaga ggaaaaaagg
     1201 aagaaaaact aaaccaccac gaccagattc cccagccact acgccaaata tatctgtgaa
     1261 gaagaaaaac aaagatggaa agggaaacac aatttatctt tgggagtttt tactggcact
     1321 gctccaggac aaggctactt gtcctaaata catcaagtgg acccagcgag agaaaggcat
     1381 ttttaaattg gtggattcta aagcagtgtc caggttgtgg gggaagcaca aaaacaaacc
     1441 tgatatgaat tatgagacca tgggaagagc actcaggtac tattaccaaa ggggtattct
     1501 ggcaaaagtg gaaggtcagc gcttggtgta tcagtttaaa gaaatgccaa aagatcttat
     1561 atatataaat gatgaggatc caagttccag catagagtct tcagatccat cactatcttc
     1621 atcagccact tcaaatagga atcaaaccag ccggtcgaga gtatcttcaa gtccaggggt
     1681 aaaaggagga gccactacag ttctaaaacc agggaattct aaagctgcaa aacccaaaga
     1741 tcctgtggaa gttgcacaac catcagaagt tttgaggaca gtgcagccca cgcagtctcc
     1801 atatcctacc cagctcttcc ggactgttca tgtagtacag ccagtacagg ctgtcccaga
     1861 gggagaagca gctagaacca gtaccatgca ggatgaaaca ttaaattctt ccgttcagag
     1921 tattaggact atacaggctc caacccaagt tccagtggtt gtgtctccta ggaatcagca
     1981 gttgcataca gtaacactcc aaacagtgcc actcacaaca gttatagcca gcacagatcc
     2041 atcagcaggt actggatctc agaagtttat tttacaagcc attccatcat cacagcccat
     2101 gacagtactg aaagaaaatg tcatgctgca gtcacaaaag gcgggctctc ctccttcaat
     2161 tgtcttgggc cctgcccagg ttcagcaggt ccttactagc aatgttcaga ccatttgcaa
     2221 tggaaccgtc agtgtggctt cctctccatc cttcagtgct actgcacctg tggtgacctt
     2281 ttctcctcgc agttcacagc tggttgctca cccacctggc actgtaatca cttcagttat
     2341 caaaactcaa gaaacaaaaa ctcttacaca ggaagtagag aaaaaggaat ctgaagatca
     2401 tttgaaagag aacactgaga aaacggagca gcagccacag ccttatgtga tggtagtgtc
     2461 cagttccaat ggatttactt ctcaggtagc tatgaaacaa aacgaactgc tggaacccaa
     2521 ctctttttac ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2581 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2641 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2701 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2761 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2821 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2881 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2941 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     3001 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3061 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     3121 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3181 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3241 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3301 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3361 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3421 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3481 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3541 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3601 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3661 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3721 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3781 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3841 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3901 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4021 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4081 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4141 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4201 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4261 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4321 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4381 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4441 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4501 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4561 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4621 ggccttttgc tggccttttg ctcacatgtt
//
