LOCUS       sequence-168330-        4812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KIF2B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KIF2B_p.I119T
FEATURES             Location/Qualifiers
     source          1..4812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2691..2790)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2807..2826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2808..2826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2831..2847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2831..2847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3278..4069
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4162..4750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4651..4670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggccagcc agttctgcct ccctgaatcc ccatgtctct cgcccctgaa
      721 acccttgaag ccacatttcg gagacatcca agagggcatc tacgtggcga tccagcgcag
      781 tgacaagcgg atccacctcg ctgtggtcac ggagatcaac agagaaaact attgggtcac
      841 ggtagagtgg gtggagaaag cagtcaaaaa aggcaagaag attgacctgg agaccatact
      901 cctgctgaat ccagctctgg actctgctga acaccccatg ccgcccccgc ccttatcccc
      961 cttggctctg gcgccctctt cggccatcag ggaccagcgt accgccacga aatgggttgc
     1021 gatgaccccc cagaaaaacc aaacagcctc aggggacagc ctggatgtga gggtccccag
     1081 caaaccttgt ctgatgaagc agaaaaagtc tccctgcctc tgggaaatcc agaaactgca
     1141 ggagcagcgg gaaaagcgca ggcggctgca gcaggagatc cgagctagac gcgccctcga
     1201 tgtcaatacc agaaacccca actacgaaat catgcacatg atcgaagagt atcgcaggca
     1261 cctggacagc agcaagatct cagtcctgga gcccccgcaa gaacatcgca tctgcgtctg
     1321 cgtgaggaag cggcctctca accagcgaga gacaaccttg aaggacctgg atatcatcac
     1381 cgtcccctcg gacaatgtgg ttatggtgca tgagtccaag caaaaggtgg acctcactcg
     1441 ctacctgcag aaccagacct tctgcttcga ccatgccttc gatgacaaag cctccaacga
     1501 gttggtgtac cagttcaccg cccagccact ggtggagtcc atcttccgca agggcatggc
     1561 cacctgcttt gcctatgggc agacgggaag tgggaagacg tacaccatgg gtggagactt
     1621 ttcaggaacg gcccaagatt gttctaaggg catttatgct ttggtggcac aggatgtctt
     1681 tctcctgctc agaaactcca catatgagaa gctggacctc aaagtctatg ggacattttt
     1741 tgagatttat gggggcaagg tgtatgattt gttgaactgg aagaagaagc tgcaagtcct
     1801 tgaggatggc aatcagcaaa tccaagtggt cgggctgcag gagaaagagg tgtgttgtgt
     1861 ggaggaagtg ctgaacctgg tggaaatagg gaatagctgt cggacttcca ggcaaacatc
     1921 tgtcaacgct cactcatcca ggagccatgc agtgttccag atcatcctga agtcaggagg
     1981 gataatgcat ggcaagtttt ccctcgttga tttagctggg aatgaaagag gagcagatac
     2041 aaccaaggcc agccggaaaa ggcagctgga aggggcagag attaacaaga gtcttctagc
     2101 cctcaaagaa tgtattctgg ctttgggtca gaacaagcct cacaccccat tcagagccag
     2161 caaactcaca ctggtgctcc gggactcctt tataggccag aactcctcca cttgcatgat
     2221 tgctaccatc tctccgggga tgacctcttg tgaaaacact ctcaacactt taagatatgc
     2281 aaacagagta aaaaaattaa atgtagatgt aaggccctac catcgtggcc actatccgat
     2341 tggacatgag gcaccaagga tgttaaaaag tcacatcgga aattcagaaa tgtcccttca
     2401 gagggatgaa tttattaaaa taccttatgt acagagtgag gagcagaaag agattgaaga
     2461 ggttgaaaca ttacccactc tgttagggaa ggataccaca atttcaggga agggatctag
     2521 ccaatggttg gaaaacatcc aggagagagc tggtggagta caccatgata ttgatttttg
     2581 cattgcccgg tctttgtcca ttttggagca gaaaattgat gctctgaccg agatccaaaa
     2641 gaaactgaaa ttattactag ctgacctcca cgtgaagagc aaggtagagt gcccaacttt
     2701 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2761 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2821 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2881 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2941 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     3001 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     3061 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     3121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     3181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     3241 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     3301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     3361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     3421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     3481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     3541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     3601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     4021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     4081 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     4141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4801 tgctcacatg tt
//
