LOCUS       sequence-16838-e        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD176.86
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(619..647)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actgatctta ctaactaact attctcattt aaattttcag agcttaaaaa tggctganat
       61 cactcacaac gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg
      121 tcgactctag accgcggttt tggcgcggat ccccgggatt ggccaaagga cccaaaggta
      181 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gagggtacct
      241 aatttgatcg ataatatgta catattttgt tattcgttcc ggtatcgtac ctccacctac
      301 cataaatccc ataccctaca tctttatcga ttttttgcaa attacgagcg ttgtaggggg
      361 cggagcgata ggtcctatag gttttggtat atcatcattc attcattcat tggtacattc
      421 atttacccac cttcctcttt ctgagcttct ctggagttct gtgcttcctt tttcccttat
      481 ctttatactg taatttttaa ctttcaggca ttgattggat cctatcgatt cgcggccgct
      541 gtacacccgg gtgccctata gtgagtcgta ttggtaccgg tagaaaaaat gagtaaagga
      601 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      661 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      721 aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa catatatata
      781 ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt
      841 gttcaatgct tctcgagata cccagatcat atgaaacagc atgacttttt cagagtgcca
      901 tgncccgaag gttatgtaca gggaaagaac tatatttttt tcaaagatga cggggaacta
      961 caagaacacg ntaagtttaa aacagntncg ggaactaacc taaccataca taattttaaa
     1021 ttttncaggg ggctgaaatc caanttttgn aanggngnan accccttggt taaaaaaaat
     1081 ccangttaaa aggntattgg aattttnaaa gaaaaaaggg aaaacatttc ttggganccc
     1141 aaattgggna anaccacctt ataaccnccc cccaaggggt naacnnccgg ggcanaaaaa
     1201 ncaaaaaaaa ngggaancca agnttgtang tttaaaacan gganttttnc caaccaaact
     1261 aanccgnant ttaatttncc aaanctcca
//
