LOCUS       sequence-16839-e        1283 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1283)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD177.66
FEATURES             Location/Qualifiers
     source          1..1283
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(622..650)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gttactgatc ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga
       61 aatcactcac aacgatggat acgctaacaa cttggaaatg aaataagctt gcatgcctgc
      121 aggtcgactc tagaccgcgg ttttggcgcg gatccccggg attggccaaa ggacccaaag
      181 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggagggta
      241 cctaatttga tcgataatat gtacatattt tgttattcgt tccggtatcg tacctccacc
      301 taccataaat cccataccct acatctttat cgattttttg caaattacga gcgttgtagg
      361 gggcggagcg ataggtccta taggttttgg tatatcatca ttcattcatt cattggtaca
      421 ttcatttacc caccttcctc tttctgagct tctctggagt tctgtgcttc ctttttccct
      481 tatctttata ctgtaatttt taactttcag gcattgattg gatcctatcg attcgcggcc
      541 gctgtacacc cgggtgccct atagtgagtc gtattggtac cggtagaaaa aatgagtaaa
      601 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      661 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      721 cttaaattta tttgcactac tggaaaacta cctgttccat gggtaagttt aaacatatat
      781 atactaacta accctgatta tttaaatttt cagccaacac ttgtcactac tttcggttat
      841 ggtcttcaat gcttcgccag atacccagat catatgaaac agcatgactt tttcaagagt
      901 gccatggccc gaaggttatg tacaggaaaa gaactaatnt tttttcaaag atgnacgggg
      961 aactaccaag aacncgtaaa gtttnaaacc gnttcggnac taactaaccn ttancatant
     1021 tttaaatttt ncaggggggc tgnaantcca agttttgaag ggngaananc cccttggtta
     1081 aaaaaaatcn aanttaaaaa gggnattgga tttttaaaga aaaaagggga aacattcntg
     1141 gggnaccccn aaattggaan ancaacntnt aacnccccac caagggnnat cccncccngg
     1201 gnnnaanaaa ccaaaaaaan ggnaancaaa ggttntaann ttnaacnngg aattttccca
     1261 ccnnaacaan ncgggattta aat
//
