LOCUS       sequence-168412-        7936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent human androgen receptor (fused to EGFP) with point 
            mutation C619Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Nazareth LV, Stenoien DL, Bingman WE 3rd, James AJ, Wu C, Zhang Y, 
            Edwards DP, Mancini M, Marcelli M, Lamb DJ, Weigel NL
  TITLE     A C619Y mutation in the human androgen receptor causes inactivation 
            and mislocalization of the receptor with concomitant sequestration 
            of SRC-1 (steroid receptor coactivator 1)
  JOURNAL   Mol Endocrinol. 1999 Dec;13(12):2065-75.
  PUBMED    10598582
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Endocrinol."; date: "1999-12"; volume: "13(12)"; pages: "2065-75"
COMMENT     Sequence Label: pEGFP-C1-AR C619Y
FEATURES             Location/Qualifiers
     source          1..7936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4724..4845
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4761..4780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4815..4834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4852..5307)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4989..5010)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5201..5220
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5334..5438
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5440..5797
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5440..5631
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5463..5483)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5648..5783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5832..6626
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5832..6626
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5886..5905)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6496..6515
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6814..6833)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6858..6905
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7234..7822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7723..7742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 atggaagtgc agttagggct gggaagggtc taccctcggc cgccgtccaa gacctaccga
     1441 ggagctttcc agaatctgtt ccagagcgtg cgcgaagtga tccagaaccc gggccccagg
     1501 cacccagagg ccgcgagcgc agcacctccc ggcgccagtt tgctgctgct gcagcagcag
     1561 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcaagag
     1621 actagcccca ggcagcagca gcagcagcag ggtgaggatg gttctcccca agcccatcgt
     1681 agaggcccca caggctacct ggtcctggat gaggaacagc aaccttcaca gccgcagtcg
     1741 gccctggagt gccaccccga gagaggttgc gtcccagagc ctggagccgc cgtggccgcc
     1801 agcaaggggc tgccgcagca gctgccagca cctccggacg aggatgactc agctgcccca
     1861 tccacgttgt ccctgctggg ccccactttc cccggcttaa gcagctgctc cgctgacctt
     1921 aaagacatcc tgagcgaggc cagcaccatg caactccttc agcaacagca gcaggaagca
     1981 gtatccgaag gcagcagcag cgggagagcg agggaggcct cgggggctcc cacttcctcc
     2041 aaggacaatt acttaggggg cacttcgacc atttctgaca acgccaagga gttgtgtaag
     2101 gcagtgtcgg tgtccatggg cctgggtgtg gaggcgttgg agcatctgag tccaggggaa
     2161 cagcttcggg gggattgcat gtacgcccca cttttgggag ttccacccgc tgtgcgtccc
     2221 actccttgtg ccccattggc cgaatgcaaa ggttctctgc tagacgacag cgcaggcaag
     2281 agcactgaag atactgctga gtattcccct ttcaagggag gttacaccaa agggctagaa
     2341 ggcgagagcc taggctgctc tggcagcgct gcagcaggga gctccgggac acttgaactg
     2401 ccgtctaccc tgtctctcta caagtccgga gcactggacg aggcagctgc gtaccagagt
     2461 cgcgactact acaactttcc actggctctg gccggaccgc cgccccctcc gccgcctccc
     2521 catccccacg ctcgcatcaa gctggagaac ccgctggact acggcagcgc ctgggcggct
     2581 gcggcggcgc agtgccgcta tggggacctg gcgagcctgc atggcgcggg tgcagcggga
     2641 cccggttctg ggtcaccctc agccgccgct tcctcatcct ggcacactct cttcacagcc
     2701 gaagaaggcc agttgtatgg accgtgtggt ggtggtgggg gtggtggcgg cggcggcggc
     2761 ggcggcggcg gcggcggcgg cggcggcggc ggcggcgagg cgggagctgt agccccctac
     2821 ggctacactc ggccccctca ggggctggcg ggccaggaaa gcgacttcac cgcacctgat
     2881 gtgtggtacc ctggcggcat ggtgagcaga gtgccctatc ccagtcccac ttgtgtcaaa
     2941 agcgaaatgg gcccctggat ggatagctac tccggacctt acggggacat gcgtttggag
     3001 actgccaggg accatgtttt gcccattgac tattactttc caccccagaa gacctgcctg
     3061 atctgtggag atgaagcttc tgggtgtcac tatggagctc tcacatgtgg aagctgcaag
     3121 gtcttcttca aaagagccgc tgaagggaaa cagaagtacc tgtgcgccag cagaaatgat
     3181 tgcactattg ataaattccg aaggaaaaat tgtccatctt gtcgtcttcg gaaatattat
     3241 gaagcaggga tgactctggg agcccggaag ctgaagaaac ttggtaatct gaaactacag
     3301 gaggaaggag aggcttccag caccaccagc cccactgagg agacaaccca gaagctgaca
     3361 gtgtcacaca ttgaaggcta tgaatgtcag cccatctttc tgaatgtcct ggaagccatt
     3421 gagccaggtg tagtgtgtgc tggacacgac aacaaccagc ccgactcctt tgcagccttg
     3481 ctctctagcc tcaatgaact gggagagaga cagcttgtac acgtggtcaa gtgggccaag
     3541 gccttgcctg gcttccgcaa cttacacgtg gacgaccaga tggctgtcat tcagtactcc
     3601 tggatggggc tcatggtgtt tgccatgggc tggcgatcct tcaccaatgt caactccagg
     3661 atgctctact tcgcccctga tctggttttc aatgagtacc gcatgcacaa gtcccggatg
     3721 tacagccagt gtgtccgaat gaggcacctc tctcaagagt ttggatggct ccaaatcacc
     3781 ccccaggaat tcctgtgcat gaaagcactg ctactcttca gcattattcc agtggatggg
     3841 ctgaaaaatc aaaaattctt tgatgaactt cgaatgaact acatcaagga actcgatcgt
     3901 atcattgcat gcaaaagaaa aaatcccaca tcctgctcaa gacgcttcta ccagctcacc
     3961 aagctcctgg actccgtgca gcctattgcg agagagctgc atcagttcac ttttgacctg
     4021 ctaatcaagt cacacatggt gagcgtggac tttccggaaa tgatggcaga gatcatctct
     4081 gtgcaagtgc ccaagatcct ttctgggaaa gtcaagccca tctatttcca cacccagtga
     4141 aaccctattt ccccacccca gctcatgccc cctttcagat gtcttctgcc tgttataact
     4201 ctgcactact cctctgcagt gccttgggga atttcctcta ttgatgtaca gtctgtcatg
     4261 aacatgttcc tgaattctat ttgctgggct ttttttttct ctttctctcc tttctttttc
     4321 ttcttccctc cctatctaac cctcccatgg caccttcaga ctttgcttcc cattgtggct
     4381 cctatctgtg ttttgaatgg tgttgtatgc ctttaaatct gtgatgatcc tcatatggcc
     4441 cagtgtcaag ttgtgcttgt ttacagcact actctgtgcc agccacacaa acgtttactt
     4501 atcttatgcc acgggaagtt tagagagcta agattatctg gggaaatcaa aacaaaaaca
     4561 agcaaacaaa aaaaaaagaa gacggaattc agacagatcc accggatcta gataactgat
     4621 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4681 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     4861 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4921 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4981 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     5041 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     5101 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     5161 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     5221 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     5281 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5401 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     5461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5701 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5761 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     5821 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5881 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5941 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     6001 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     6061 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     6121 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     6181 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6241 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6301 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     6361 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6421 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6481 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6541 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6601 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6661 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6781 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     6841 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     6901 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6961 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     7021 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     7081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7381 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7921 tattaccgcc atgcat
//
