LOCUS       sequence-16842-e        1255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD176.36
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(246..274)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gntcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggtcg
      121 actctagacc gcggttttgg cgcggatcct atcgattcgc ggccgctgta cacccgggtg
      181 ccctatagtg agtcgtattg gtaccggtag aaaaaatgag taaaggagaa gaacttttca
      241 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      301 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      361 ctactggaaa actacctgtt ccatgggtaa gtttaaacat atatatacta actaaccctg
      421 attatttaaa ttttcagcca acacttgtca ctactttctg ttatggtgtt caatgcttct
      481 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      541 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtaagttta
      601 aacagttcgg tactaactaa ccatacatat ttaaattttc aggtgctgaa gtcaagtttg
      661 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      721 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      781 acaaacaaaa gaatggaatc aaagttgtaa gtttaaacat gattttacta actaactaat
      841 ctgatttaaa ttttcagaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      901 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtccnt tttaccagac
      961 aaccattacc tgtccncnca atctgcccnt tcnaaagatc ccaacgaaaa gagagaccnc
     1021 atggtccctt ctggagtttg taacnnctgc tgggattacc cntggccagg gacgaactat
     1081 acnaaaagcc ccnnaagcnt ttggaaatnc cngccngccc ngggggannc ctgggnggaa
     1141 acccngtaac ntttanacna ngttccccaa aaaaatgccc ggngntctnc ttnatcaaaa
     1201 ccnntttccc ncnggggggg aancnnggcc nnccccnttt tctgccaaaa ncccg
//
