LOCUS       sequence-16844-e        1273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1273)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD187.49
FEATURES             Location/Qualifiers
     source          1..1273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    27..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(433..452)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tnctgagtcg aggtatacag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc
      181 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
      241 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
      301 tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
      361 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
      421 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
      481 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
      541 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
      601 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
      661 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
      721 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
      781 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
      841 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gnaacgcccg
      901 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggnac
      961 aggntcggtc ttgacaaaaa gaaccgggcg ccccctgcnc tgacagccgg naacacggng
     1021 ggcatcaaan caaccnnatt gtcttgttgt gccccagtcc atanccgaaa tanccctctc
     1081 ccncccaagn nggccggaaa aacctngggn ggcaatcccn tcttggttca atcatggnaa
     1141 aannaatccc ccannccngg tccncttgna tcnaaatccn aaaaatggga annacaagnc
     1201 tccccggggg ancttttttn nnaaaaagcc ctaggnccnc ccaaaaaaan ccnncctncc
     1261 tnntttttng gaa
//
