LOCUS       sequence-168442-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses (untagged) Histamine-2 receptor and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   van Unen J, Rashidfarrokhi A, Hoogendoorn E, Postma M, Gadella TW 
            Jr, Goedhart J
  TITLE     Quantitative Single-Cell Analysis of Signaling Pathways Activated 
            Immediately Downstream of Histamine Receptor Subtypes.
  JOURNAL   Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. 
            Epub 2016 Jun 29.
  PUBMED    27358232
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1124/mol.116.104505"; journalName: "Mol Pharmacol"; date: 
            "2016-09"; volume: "90"; issue: "3"; pages: "162-76"
COMMENT     Sequence Label: pH2R-P2A-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(127..183)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      121 ggacccaggt ccagggttct cctccacgtc tccagcctgc ttcagcaggc tgaagttagt
      181 agcacccgat cccaccggtg gatcccgggc ccgcggcctg tctgtggctc cctggggggc
      241 cgtgacttct gtcccactcc acacctggag cttcaggggt ttctcttcct gttgcctggg
      301 ttctcggctt tgggtcctgg acagctgaga ggcgttggac ctcagagaag ttttgtggga
      361 gttgcggttg gccagcctgc agcagaagag ctgttggtac ccggtgcgga agtctctgtt
      421 cagcgcagca tacaggatgg ggttcagggc tgagttggca tagcccagcc acagaacgat
      481 ggcttctaac acctcattga tggcatcatc ccctctcagc ccacggtaca caaacgcggt
      541 gaagtaggga aaccagcaga tgatgaaggc ccccatgacg gcggccagtg tcactgtggc
      601 tttgtgctcc ctgatggtgg ctgccttcca ggagctaatg tgattgatcc tcttggcctg
      661 atcccgggcg accttgaaga tgcggtagta ggtgatgcac atgatcagta gcgggaggta
      721 gaaggtgacc agcccatcca ccagcccgta cacttcattg acctggactt tgcacttaga
      781 ggtggtatga ttgcccttgc tggtctcgtt cctgctgttc caccccaggt ggatagacag
      841 aaaggacagg gtaatgg
//
