LOCUS       sequence-168445-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables
            bicistronic expression of protein of interest and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   van Unen J, Rashidfarrokhi A, Hoogendoorn E, Postma M, Gadella TW 
            Jr, Goedhart J
  TITLE     Quantitative Single-Cell Analysis of Signaling Pathways Activated 
            Immediately Downstream of Histamine Receptor Subtypes.
  JOURNAL   Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. 
            Epub 2016 Jun 29.
  PUBMED    27358232
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1124/mol.116.104505"; journalName: "Mol Pharmacol"; date: 
            "2016-09"; volume: "90"; issue: "3"; pages: "162-76"
COMMENT     Sequence Label: pP2A-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..105
                     /label=MCS
                     /note="multiple cloning site"
     CDS             116..172
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(324..342)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     592..611
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtgggat cgggtgctac
      121 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctgggtccat
      181 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      241 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      301 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
      361 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
      421 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      481 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      541 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      601 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      661 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      721 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
      781 catcaagttg gacatcacct cccac
//
