LOCUS       sequence-168446-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables
            bicistronic expression of protein of interest and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   van Unen J, Rashidfarrokhi A, Hoogendoorn E, Postma M, Gadella TW 
            Jr, Goedhart J
  TITLE     Quantitative Single-Cell Analysis of Signaling Pathways Activated 
            Immediately Downstream of Histamine Receptor Subtypes.
  JOURNAL   Mol Pharmacol. 2016 Sep;90(3):162-76. doi: 10.1124/mol.116.104505. 
            Epub 2016 Jun 29.
  PUBMED    27358232
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1124/mol.116.104505"; journalName: "Mol Pharmacol"; date: 
            "2016-09"; volume: "90"; issue: "3"; pages: "162-76"
COMMENT     Sequence Label: pP2A-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(410..429)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     679..697
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 taacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
       61 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag
      121 tcgcggccgc tttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
      181 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
      241 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
      301 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
      361 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
      421 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
      481 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
      541 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
      601 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
      661 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc
//
