LOCUS       sequence-168454-        3528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ISX , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ISX_p.T87A
FEATURES             Location/Qualifiers
     source          1..3528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(928..1027)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1044..1063)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1045..1063)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1068..1084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1068..1084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1515..2306
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2399..2987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2888..2907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3141..3158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3317..3344)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3436..3522)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgacaact ttgtacaaaa
      181 aagttggcac catgtgtgct gaggtgggcc ctgctctctg caggggtatg gagagaaata
      241 gcttggggtg ctgtgaggcc ccgaagaagc tgggcctgtc cttctccatc gaggcgatcc
      301 taaagaggcc tgccaggagg agtgatatgg acagaccaga agggccaggt gaagagggct
      361 ccggagaagc tgcggcctca ggctctgggc tagaaaagcc tccaaaggac cagccccagg
      421 aaggaaggaa gagcaagcgg agggttcgtg ccaccttcac cactgagcag ctgcatgagc
      481 tggagaagat cttccacttt acccactacc cagacgttca catccgcagc cagctggcag
      541 ccaggatcaa cctcccagaa gctcgggtgc agatctggtt ccagaatcag cgagccaagt
      601 ggcggaagca ggagaagatt ggcaacctgg gggctccaca gcagctgagt gaagccagtg
      661 tggccctgcc cacaaatctg gatgtggctg ggcccacgtg gacatccact gctctgcgca
      721 ggctggctcc tcccacgagc tgttgtccat cggctcaaga tcagctggcc tctgcctggt
      781 tccctgcctg gatcaccctc ctcccagcgc acccatggga aacacagcct gtcccaggtc
      841 ttcccatcca tcaaacttgc atccctgtgc tatgcatcct tccacctcca caccccaaat
      901 ggggcagcat ctgtgctact tcaacattgc caactttctt gtacaaagtt ggcattataa
      961 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1021 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1081 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1141 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1201 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1261 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1321 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1381 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1441 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1501 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1561 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1621 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     1681 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1741 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1801 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1861 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1921 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1981 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2041 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2101 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2161 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2221 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2281 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2341 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2401 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2461 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2521 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2581 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2641 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2701 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2761 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2821 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2881 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2941 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3001 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3061 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3181 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3241 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3301 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3361 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     3421 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3481 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
