LOCUS       sequence-168464-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducibile Rpa* Promoter driving sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs074-Prpa*
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             217..225
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             232..942
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
ORIGIN
        1 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
       61 gtacaaaaaa gcaggctccg aattggtatc acgaggcaga atttcagata aaaaaaatcc
      121 ttagctttcg ctaaggatct gaagtggaat tcatgagatc tgcacctgtc cgatcggaca
      181 gtattacgca agaaaatggt ttgttatagt cgaatataag gaggtgacaa tatgcgtaaa
      241 ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg tgatgtcaac
      301 ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg taaactgacg
      361 ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct ggtaacgacg
      421 ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca gcatgacttc
      481 ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt taaggatgac
      541 ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt
      601 gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa gctggaatac
      661 aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg cattaaagcg
      721 aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga tcactaccag
      781 caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta tctgagcacg
      841 caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct gctggagttc
      901 gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaa
//
