LOCUS       sequence-168472-        3579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GZMA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GZMA_p.G210E
FEATURES             Location/Qualifiers
     source          1..3579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(979..1078)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1095..1114)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1096..1114)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1119..1135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1119..1135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1566..2357
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2450..3038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2939..2958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3192..3209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3368..3395)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3487..3573)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggtat gaggaactcc aatagatttc tggcatcctc tctctcagtt gtcgtttctc
      241 tcctgctaat tcctgaagat gtctgtgaaa aaattattgg aggaaatgaa gtaactcctc
      301 attcaagacc ctacatggtc ctacttagtc ttgacagaaa aaccatctgt gctggggctt
      361 tgattgcaaa agactgggtg ttgactgcag ctcactgtaa cttgaacaaa aggtcccagg
      421 tcattcttgg ggctcactca ataaccaggg aagagccaac aaaacagata atgcttgtta
      481 agaaagagtt tccctatcca tgctatgacc cagccacacg cgaaggtgac cttaaacttt
      541 tacagctgac ggaaaaagca aaaattaaca aatatgtgac tatccttcat ctacctaaaa
      601 agggggatga tgtgaaacca ggaaccatgt gccaagttgc agggtggggc aggactcaca
      661 atagtgcatc ttggtccgat actctgagag aagtcaatat caccatcata gacagaaaag
      721 tctgcaatga tcgaaatcac tataatttta accctgtgat tggaatgaat atggtttgtg
      781 ctggaagcct ccgaggtgga agagactcgt gcaatgaaga ttctggaagc cctttgttgt
      841 gcgagggtgt tttccgaggg gtcacttcct ttggccttga aaataaatgc ggagaccctc
      901 gtgggcctgg tgtctatatt cttctctcaa agaaacacct caactggata attatgacta
      961 tcaagggagc agtttgattg ccaactttct tgtacaaagt tggcattata agaaagcatt
     1021 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1081 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1141 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1201 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1261 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1321 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1381 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1441 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1501 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1561 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1621 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1681 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1741 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1801 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1861 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1921 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1981 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2041 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2101 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2161 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2221 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2281 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2341 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2401 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3301 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3361 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3421 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3481 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3541 ccagtcttcc gactgagcct ttcgttttat ttgatgcct
//
