LOCUS       sequence-168484-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter driven by a DNA damage responsive cis-regulatory
            sequence from the reaper locus, containing a p53 binding site 
            (Rpr150 enhancer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pRpr150-LUC
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      425..434
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(497..515)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc ttacatacat actagaattc cgtccgctcg acttgttcaa
       61 acatgtcagg ttggttcttc cacttttatt tgagtaattt tcgccctttt tccatagatt
      121 ttcatagata acgaattttc ggaatgggtt ttcagatttc ttcactcagc gtgcatcgac
      181 gtgttcgaag gatcctctag agtcgacctc gaacgttaac gttaacgtaa cgttaactcg
      241 aggagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
      301 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
      361 ctaaacaatc tgcagccaag ctgatcctct agggcaagct tggcattccg gtactgttgg
      421 taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      481 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      541 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      601 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      661 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      721 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      781 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
//
