LOCUS       sequence-168485-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter driven by a DNA damage responsive cis-regulatory
            sequence from the reaper locus, containing a p53 binding site 
            (Rpr150 enhancer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Mauri F, McNamee LM, Lunardi A, Chiacchiera F, Del Sal G, Brodsky 
            MH, Collavin L
  TITLE     Modification of Drosophila p53 by SUMO modulates its transactivation
            and pro-apoptotic functions.
  JOURNAL   J Biol Chem. 2008 Jul 25. 283(30):20848-56.
  PUBMED    18492669
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jul 25. 283(30):20848-56."
COMMENT     Sequence Label: pRpr150-LUC
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    462..553
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(494..513)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    567..615
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttgccctag aggatcagct tggctgcaga ttgtttagct tgttcagctg
      121 cgcttgttta tttgcttagc tttcgcttag cgacgtgttc actttgcttg tttgaattga
      181 attgtcgctc cgtagacgaa gcgcctctat ttatactccg gcgctcctcg agttaacgtt
      241 acgttaacgt taacgttcga ggtcgactct agaggatcct tcgaacacgt cgatgcacgc
      301 tgagtgaaga aatctgaaaa cccattccga aaattcgtta tctatgaaaa tctatggaaa
      361 aagggcgaaa attactcaaa taaaagtgga agaaccaacc tgacatgttt gaacaagtcg
      421 agcggacgga attctagtat gtatgtaagg tacctatcga tagagaaatg ttctggcacc
      481 tgcacttgca ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg
      541 gagagcgtat gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa
      601 taaagatatt ttattgcggc cgctccaagt acctcccgta ccttaatatt acttacttat
      661 catggtagct tgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      721 ttgcgcagcc tgaatggcga atggcaaatt gtaagcgtta atattttgtt aaaattcgcg
      781 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaa
//
