LOCUS       sequence-168488-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses LuxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-LuxR
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     159..176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(209..236)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(252..323)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatta
       61 ttttttataa tgccaacttt gtacaagaaa gctgggtcga attgattacc gcctttgagt
      121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      181 cctgaggagc gagttaggat cctataaacg cagaaaggcc cacccgaagg tgagccagtg
      241 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      301 gactgagcct ttcgttttat ttgatgcctg gagatcctta ctcgagtttg gatccttaat
      361 ttttaaagta tgggcaatca attgctcctg ttaaaattgc tttagaaata ctttggcagc
      421 ggtttgttgt attgagtttc atttgcgcat tggttaaatg gaaagtgaca gtacgctcac
      481 tgcagcctaa tatttttgaa atatcccaag agctttttcc ttcgcatgcc cacgctaaac
      541 attctttttc tcttttggtt aaatcgttgt ttgatttatt atttgctata tttatttttc
      601 gataattatc aactagagaa ggaacaatta atggtatgtt catacacgca tgtaaaaata
      661 aactatctat atagttgtct ttttctgaat gtgcaaaact aagcattccg aagccattgt
      721 tagccgtatg aatagggaaa ctaaacccag tgataagacc tgatgttttc gcttctttaa
      781 ttacatttgg agatttttta tttacagcat tgttttcaaa tatattccaa ttaattggtg
      841 aatgattgga gttagaataa tctactatag gatcatattt tattaaatta gcgtcatcat
      901 aatattgcct ccatttttta gggtaattat ctagaattga aatatcagat
//
