LOCUS       sequence-168496-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses RpaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-RpaR
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(333..338,339..355,356..362)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa
       61 aaaagcaggc tccgaattgg tatcacgagg cagaatttca gataaaaaaa atccttagct
      121 ttcgctaagg atctgaagtg gaattcatga gatctgaatt caaaagatct taagtaagta
      181 agagtatacg tatatcggct aataacgtat taaggcgctt cggcgccttt ttttatgggg
      241 gtattttcat cccaatccac acgtccaacg cacagcaaac accacgtcga ccctatcagc
      301 tgcgtgcttt ctatgagtcg ttgctgcata acttgacaat taatcatccg gctcgtataa
      361 tgtgtggaag ggcccaagtt cacttaaaaa ggagatcaac aatgaaagca attttcgtac
      421 tgaaacatct taatcatgcg atggacggtt tctaatgatc gtcggcgaag atcagctttg
      481 gggacggcgt gcgctggaat tcgtcgattc cgtcgaacgg ctcgaggcgc cggcgctgat
      541 cagccggttc gaatcgctga tcgcgagctg cggatttacc gcctacatca tggccggcct
      601 gccgtcgcgc aatgccggac taccggagct gacgctggcc aatggctggc cgcgagactg
      661 gttcgatctg tatgtcagcg aaaacttca
//
