LOCUS       sequence-168497-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses RpaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-RpaR
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     151..168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(201..228)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(244..315)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat tattttttat
       61 aatgccaact ttgtacaaga aagctgggtc gaattgatta ccgcctttga gtgagctgat
      121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgagga
      181 gcgagttagg atcctataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta
      241 gtagagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
      301 ctttcgtttt atttgatgcc tggagatcct tactcgagtt tggatcctca caaacggatc
      361 aatccgagcg tcaatgcctt ggcaaccgcc gcggtgcggt tggcggcgtc gagcttgcgg
      421 gcggcttcga tcagatggaa tttcaccgtg cgttcggtga tgcagaggat taccgagatt
      481 tcccaggcgg tcttgccctg cgctgcccat tgcaggatct cgcactcgcg cggcgtgagc
      541 cggttgcgcc ggatcggctt tggccggctg agtgcgcgca ggcgactatg cgcgtagatg
      601 ctgaccagct gcatcgcgcc gcgcgcggcc gggctgaggt ccggatcctt gccggccatg
      661 ctgatcgcgg cgctaccgtc gtcgtagtgc a
//
