LOCUS       sequence-168505-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses TraR(W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-TraR(W)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(340..345,346..362,363..369)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agcaggctcc gaattggtat cacgaggcag aatttcagat aaaaaaaatc
      121 cttagctttc gctaaggatc tgaagtggaa ttcatgagat ctgaattcaa aagatcttaa
      181 gtaagtaaga gtatacgtat atcggctaat aacgtattaa ggcgcttcgg cgcctttttt
      241 tatgggggta ttttcatccc aatccacacg tccaacgcac agcaaacacc acgtcgaccc
      301 tatcagctgc gtgctttcta tgagtcgttg ctgcataact tgacaattaa tcatccggct
      361 cgtataatgt gtggaagggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt
      421 ttcgtactga aacatcttaa tcatgctggg gagggtttct aatgcagcac tggctggata
      481 agctgactga tctggcggca attgagggtg atgaatgcat tctgaaaact ggtctggcgg
      541 atattgcgga ccacttcggt tttaccggtt acgcgtacct gcacatccag caccgtcata
      601 ttaccgctgt gaccaactac caccgtcaat ggcaatccac ctacttcgat aaaaaattcg
      661 aggcgctgga cccggtggtt aagcgtgcgc gttctcgcaa acacatcttc acttggagcg
      721 gtgaacacga acgtccaact ctgagcaaag acgaacgcgc gttctacgac cacgcgtccg
      781 actttggtat ccgttctggc atcaccatt
//
