LOCUS       sequence-168506-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses TraR(W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-TraR(W)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     159..176
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(209..236)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(252..323)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatta
       61 ttttttataa tgccaacttt gtacaagaaa gctgggtcga attgattacc gcctttgagt
      121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      181 cctgaggagc gagttaggat cctataaacg cagaaaggcc cacccgaagg tgagccagtg
      241 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
      301 gactgagcct ttcgttttat ttgatgcctg gagatcctta ctcgagtttg gatcctcaga
      361 tcagcttgcg acggatagcc agggccgtca ggtgggcttt agaacgtacg tcgaagcgtt
      421 tcatagcttc gcgcagtttt acgcggacgg aattgtattt aacgccttcg atatctgcga
      481 tttcccacat ggttttgcct acggcgatcc aacgcaggta agtagcttcc ttaggatcca
      541 gccatgcagc gtcttccgcc gtcggggtcg tgcgcagaaa ggagatacgc gcgtgaattt
      601 gaccgatggt cgccgccgca gctacggcgt cgatttcacg gtccaggtcg ataaccggtt
      661 tatcgcttgc catggtgaac atagacatga aaccgttggc cgttttaatc ggaatggtga
      721 tgccagaacg gataccaaag tcggacgcgt ggtcgtagaa cgcgcgttcg tctttgctca
      781 gagttggacg ttcgtgttca ccgctccaag tgaagatgtg tttgcgagaa cgcgcacgct
      841 taaccaccgg gtccagcgcc tcgaattttt tatcgaagta ggtggattgc cattgacggt
      901 g
//
