LOCUS       sequence-168508-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses TraR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-TraR
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(338..343,344..360,361..367)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             741..752
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
       61 gtacaaaaaa gcaggctccg aattggtatc acgaggcaga atttcagata aaaaaaatcc
      121 ttagctttcg ctaaggatct gaagtggaat tcatgagatc tgaattcaaa agatcttaag
      181 taagtaagag tatacgtata tcggctaata acgtattaag gcgcttcggc gcctttttta
      241 tgggggtatt ttcatcccaa tccacacgtc caacgcacag caaacaccac gtcgacccta
      301 tcagctgcgt gctttctatg agtcgttgct gcataacttg acaattaatc atccggctcg
      361 tataatgtgt ggaagggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt
      421 cgtactgaaa catcttaatc atgctgggga gggtttctaa tgcagcactg gctggacaag
      481 ttgaccgatc ttgccgcaat tcagggcgac gagtgcatcc tgaaggatgg ccttgccgac
      541 cttgccgaac atttcggctt caccggctat gcctatctcc atatccagca caaacacacc
      601 atcgcggtca ccaattatca tcgtgactgg cgatcggctt acttcgagaa caacttcgac
      661 aagctcgatc cggtcgtcaa gcgcgcgaaa tccaggaagc acgtctttgc ctggtccggc
      721 gaacaggaac gatcgcggct atcgaaggaa gagcgtgcct tctacgcgca tgcggccgat
      781 ttcggcatcc gctccggcat caccattccg atcaagaccg ccaacggatc aatgtcgatg
      841 ttcacgctgg cgtc
//
