LOCUS       sequence-168509-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses TraR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-TraR
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(204..231)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(247..318)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(763..774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aattattttt
       61 tataatgcca actttgtaca agaaagctgg gtcgaattga ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctga
      181 ggagcgagtt aggatcctat aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact
      241 ctagtagaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg
      301 agcctttcgt tttatttgat gcctggagat ccttactcga gtttggatcc ttagatcagc
      361 tttcttctga ttgcgagggc ggtgagatgg gccttgctgc gaacgtcgaa gcgcttcatg
      421 gcctcgcgga gcttgacacg gacgctgttg tacttgacgc cctccacgtc tgcgacttcc
      481 tccattgtca tgccgacggc gatccatctg agataggtcg cctctttcgg atcgagccag
      541 gcggcatctt ccactgtcgg agtggtctga aggaaagaga tgcgggcatg gagctgcccg
      601 acggcgcccg cggctgcggc cgcgtcgatc tcacggtcga ggtcgatcgc cggcctttcc
      661 gacgccagcg tgaacatcga cattgatccg ttggcggtct tgatcggaat ggtgatgccg
      721 gagcggatgc cgaaatcggc cgcatgcgcg tagaaggcac gctcttcctt cgatagccgc
      781 gatcgttcct gttcgccgga ccaggcaaag acgtgcttcc tggatt
//
