LOCUS       sequence-168510-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses LasR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-LasR
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(338..343,344..360,361..367)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
       61 gtacaagaaa gctgggtcga attggtatca cgaggcagaa tttcagataa aaaaaatcct
      121 tagctttcgc taaggatctg aagtggaatt catgagatct gaattcaaaa gatcttaagt
      181 aagtaagagt atacgtatat cggctaataa cgtattaagg cgcttcggcg ccttttttta
      241 tgggggtatt ttcatcccaa tccacacgtc caacgcacag caaacaccac gtcgacccta
      301 tcagctgcgt gctttctatg agtcgttgct gcataacttg acaattaatc atccggctcg
      361 tataatgtgt ggaagggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt
      421 cgtactgaaa catcttaatc atgctgggga gggtttctaa tggccttggt tgacggtttt
      481 cttgagctgg aacgctcaag tggaaaattg gagtggagcg ccatcctgca gaagatggcg
      541 agcgaccttg gattctcgaa gatcctgttc ggcctgttgc ctaaggacag ccaggactac
      601 gagaacgcct tcatcgtcgg caactacccg gccgcctggc gcgagcatta cgaccgggct
      661 ggctacgcgc gggtcgaccc gacggtcagt cactgtaccc agagcgtact gccgattttc
      721 tgggaaccgt ccatctacca gacgcgaaag cagcacgagt tcttcgagga agcctcggcc
      781 gccggcctgg tgtatgggct gaccatgccg ctgcatggtg ctcgcggcga actcggcgcg
      841 ctgagcctca gcgtggaagc ggaaaaccgg gccgaggcca accgtttcat ggagtcggtc
      901 ctgccgaccc tgtggatgct caaggactac gcactgcaga
//
