LOCUS       sequence-168511-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses LasR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-LasR
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     156..173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(206..233)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(249..320)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaattattt
       61 tttataatgc caactttgta caagaaagct gggtcgaatt gattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcct
      181 gaggagcgag ttaggatcct ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
      241 ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      301 tgagcctttc gttttatttg atgcctggag atccttactc gagtttggat cctcagagag
      361 taataagacc caaattaacg gccataatgg ccgctacgcg gcgggaggtc acaccgaact
      421 tccgccgaat atttcccata tggaagttca cattggcttc cgagcagttg cagataaccg
      481 atatctccca actggtcttg ccgatggcgc accactgcaa cacttccttc tcccggctgg
      541 tcagaaccac cggtttgctg accggatgtt cgaaggccag tccggcaccg ctctgcagtg
      601 cgtagtcctt gagcatccac agggtcggca ggaccgactc catgaaacgg ttggcctcgg
      661 cccggttttc cgcttccacg ctgaggctca gcgcgccgag ttcgccgcga gcaccatgca
      721 gcggcatggt cagcccatac accaggccgg cggccgaggc ttcctcgaag aactcgtgct
      781 gctttcgcgt ctggtagatg gacggttccc agaaaatcgg cagtacgctc tgggtacagt
      841 gactgaccgt cgggtcgacc cgcgcgtagc
//
