LOCUS       sequence-168530-         337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Titration sponge for TetR and cI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT149
FEATURES             Location/Qualifiers
     source          1..337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             121..132
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(142..167,168..173,174..190,191..196,
                     197..215))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    172..190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(236..254)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctagcacgcg taccatggga tctcaccgcc agaggtaaaa tagtcaacac gcacggtgtt
       61 atcaagctta ttaaactgat gcagcgtagt tttcgtcgtt tgctgcaggc ctgcggtacc
      121 tttctcctct ttaatgaatt cggtcagtgc gtcctgctga tgtgctcagt atctctatca
      181 ctgataggga tgtcaatctc tatcactgat agggactcga ggtgaagacg aaagggcctc
      241 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcggaa
      301 ttgccagctg gggcgccctc tggtaaggtt gggaagc
//
