LOCUS       sequence-168531-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Titration sponge for TetR and cI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT149
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    23..41
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             81..92
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      219..305
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(550..569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctatcagtga tagagattga catccctatc agtgatagag atactgagca catcagcagg
       61 acgcactgac cgaattcatt aaagaggaga aaggtaccgc aggcctgcag caaacgacga
      121 aaactacgct gcatcagttt aataagcttg ataacaccgt gcgtgttgac tattttacct
      181 ctggcggtga gatcccatgg tacgcgtgct agaggcatca aataaaacga aaggctcagt
      241 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      301 caaatccgcc gccctagacc tagggcgttc ggctgcggcg agcggtatca gctcactcaa
      361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      901 acactaga
//
