LOCUS       sequence-168536-         577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Titration sponge for TetR, cI and lacI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT145
FEATURES             Location/Qualifiers
     source          1..577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             329..340
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(350..375,376..381,382..398,399..404,
                     405..423))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    380..398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    405..423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctctagcacg cgtaccatgg gatcttgtta tccgctcaca atgtcaattc tcgaggtgaa
       61 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      121 cttagacgtc aggtggcact tttcgggtca caaaagacat tccacttgtt atccgctcac
      181 aacaaacgtc gtggcggctg gcgttccttg gataaatatc taacaccgtg cgtgttgact
      241 attttacctc tggcggtgat aatggttgct caagcttatt aaactgatgc agcgtagttt
      301 tcgtcgtttg ctgcaggcct gcggtacctt tctcctcttt aatgaattcg gtcagtgcgt
      361 cctgctgatg tgctcagtat ctctatcact gatagggatg tcaatctcta tcactgatag
      421 ggactcgagg tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      481 gataataatg gtttcttaga cgtcggaatt gccagctggg gcgccctctg gtaaggttgg
      541 gaagccctgc aaagtaaact ggatggcttt cttgccg
//
