LOCUS       sequence-168537-         538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Titration sponge for TetR, cI and lacI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Potvin-Trottier L, Lord ND, Vinnicombe G, Paulsson J
  TITLE     Synchronous long-term oscillations in a synthetic gene circuit.
  JOURNAL   Nature. 2016 Oct 12;538(7626):514-517. doi: 10.1038/nature19841.
  PUBMED    27732583
REFERENCE   2  (bases 1 to 538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-12- 12"; volume: "538"; issue: "7626"; 
            pages: "514-517"
COMMENT     Sequence Label: pLPT145
FEATURES             Location/Qualifiers
     source          1..538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(42..61)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
       61 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      361 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
      421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaa
//
