LOCUS       sequence-168575-        4984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Mycobacterium tuberculosis FGE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Carrico IS, Carlson BL, Bertozzi CR
  TITLE     Introducing genetically encoded aldehydes into proteins.
  JOURNAL   Nat Chem Biol. 2007 Jun . 3(6):321-2.
  PUBMED    17450134
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 Jun . 3(6):321-2."
COMMENT     Sequence Label: pBAD/myc-his A Rv0712 (FGE)
FEATURES             Location/Qualifiers
     source          1..4984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     179..201
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(357..1235)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1262..1546
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1469..1488
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2528..2557
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2573..2590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2646..2663)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2816..2902
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2994..3021
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3040..3131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3132..3200,3201..3992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3350..3369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4163..4751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4652..4671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4905..4922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    join(4937..4984,1..93)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
       61 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
      121 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      181 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc
      241 aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg
      301 attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat
      361 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
      421 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
      481 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
      541 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
      601 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
      661 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
      721 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
      781 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
      841 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
      901 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
      961 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
     1021 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
     1081 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     1141 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     1201 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
     1261 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
     1321 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
     1381 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
     1441 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
     1501 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gttttttggg
     1561 ctaacaggag gaattaacca tgctgaccga gttggttgac ctgcccggcg gatcgttccg
     1621 catgggctcg acgcgcttct accccgaaga agcgccgatt cataccgtga ccgtgcgcgc
     1681 ctttgcggta gagcgacacc cggtgaccaa cgcgcaattt gccgaattcg tctccgcgac
     1741 aggctatgtg acggttgcag aacaacccct tgaccccggg ctctacccag gagtggacgc
     1801 agcagacctg tgtcccggtg cgatggtgtt ttgtccgacg gccgggccgg tcgacctgcg
     1861 tgactggcgg caatggtggg actgggtacc tggcgcctgc tggcgccatc cgtttggccg
     1921 ggacagcgat atcgccgacc gagccggcca cccggtcgta caggtggcct atccggacgc
     1981 cgtggcctac gcacgatggg ctggtcgacg cctaccgacc gaggccgagt gggagtacgc
     2041 ggcccgtggc ggaaccacgg caacctatgc gtggggcgac caggagaagc cggggggcat
     2101 gctcatggcg aacacctggc agggccggtt tccttaccgc aacgacggtg cattgggctg
     2161 ggtgggaacc tccccggtgg gcaggtttcc ggccaacggg tttggcttgc tcgacatgat
     2221 cggaaacgtt tgggagtgga ccaccaccga gttctatcca caccatcgca tcgatccacc
     2281 ctcgacggcc tgctgcgcac cggtcaagct cgctacagcc gccgacccga cgatcagcca
     2341 gaccctcaag ggcggctcgc acctgtgcgc gccggagtac tgccaccgct accgcccggc
     2401 ggcgcgctcg ccgcagtcgc aggacaccgc gaccacccat atcgggttcc ggtgcgtggc
     2461 cgacccggtg tccgggtagc tcgaggatct gcagctggta ccatatggga attcgaagct
     2521 tgggcccgaa caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca
     2581 tcatcatcat tgagtttaaa cggtctccag cttggctgtt ttggcggatg agagaagatt
     2641 ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
     2701 ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     2761 agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     2821 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2881 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     2941 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
     3001 catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac
     3061 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3361 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     4921 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4981 accg
//
