LOCUS       sequence-168583-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mVenus tagged with a membrane localization sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   van Unen J, Botman D, Yin T, Wu YI, Hink MA, Gadella TWJ Jr, Postma 
            M, Goedhart J
  TITLE     The C-terminus of the oncoprotein TGAT is necessary for plasma 
            membrane association and efficient RhoA-mediated signaling.
  JOURNAL   BMC Cell Biol. 2018 Jun 7;19(1):6. doi: 10.1186/s12860-018-0155-2.
  PUBMED    29879899
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2018-06-7"; volume: "19(1):6. doi"; pages: " 
            10.1186/s12860-018-0155-2"
COMMENT     Sequence Label: pLck-mVenus-C1
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    125..190
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(191..442)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(233..254)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     582..601
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
       61 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagatc
      121 cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cgaagcttga gctcgagatc
      181 tgagtccgga cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      241 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc
      301 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      361 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
      421 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
      481 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      541 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      601 ccttgaagaa gatggtgcgc tcctggacgt
//
