LOCUS       sequence-168584-        5546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Lowry WE, Richter L, Yachechko R, Pyle AD, Tchieu J, Sridharan R, 
            Clark AT, Plath K
  TITLE     Generation of human induced pluripotent stem cells from dermal 
            fibroblasts.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8.
  PUBMED    18287077
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Feb 26. 105(8):2883-8."
COMMENT     Sequence Label: pMXs-hNANOG (Plath)
FEATURES             Location/Qualifiers
     source          1..5546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(986..1777,1778..1846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1609..1628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1847..1951)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1981..2574
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2637..2994
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3053..3469
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3399..3421
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3437..3453
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3479..3853
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3791..3807
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             5010..5479
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta
      961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat
     1981 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     2041 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
     2101 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     2161 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     2221 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     2281 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     2341 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     2401 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
     2461 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     2521 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     2581 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     2641 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
     2701 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     2761 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
     2821 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
     2881 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     2941 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     3001 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     3061 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     3121 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     3181 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     3241 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     3301 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     3361 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     3421 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     3481 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     3541 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     3601 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     3661 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     3721 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     3781 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     3841 atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt ggtacgggaa
     3901 ttcatgagtg tggatccagc ttgtccccaa agcttgcctt gctttgaagc atccgactgt
     3961 aaagaatctt cacctatgcc tgtgatttgt gggcctgaag aaaactatcc atccttgcaa
     4021 atgtcttctg ctgagatgcc tcacacggag actgtctctc ctcttccctc ctccatggat
     4081 ctgcttattc aggacagccc tgattcttcc accagtccca aaggcaaaca acccacttct
     4141 gcagagaata gtgtcgcaaa aaaggaagac aaggtcccag tcaagaaaca gaagaccaga
     4201 actgtgttct cttccaccca gctgtgtgta ctcaatgata gatttcagag acagaaatac
     4261 ctcagtctcc agcagatgca agaactctcc aacatcctga acctcagcta caaacaggtg
     4321 aagacctggt tccagaacca gagaatgaaa tctaagaggt ggcagaaaaa caactggccg
     4381 aagaatagca atggtgtgac gcagaaggcc tcagcaccta cctaccccag cctctactct
     4441 tcctaccacc agggatgcct ggtgaacccg actgggaacc ttccaatgtg gagcaaccag
     4501 acctggaaca attcaacctg gagcaaccag acccagaaca tccagtcctg gagcaaccac
     4561 tcctggaaca ctcagacctg gtgcacccaa tcctggaaca atcaggcctg gaacagtccc
     4621 ttctataact gtggagagga atctctgcag tcctgcatgc agttccagcc aaattctcct
     4681 gccagtgact tggaggctgc tttggaagct gctggggaag gccttaatgt aatacagcag
     4741 accactaggt attttagtac tccacaaacc atggatttat tcctaaacta ctccatgaac
     4801 atgcaacctg aagacgtgtg agcggccgcc agcacagtgg tcgacgataa aataaaagat
     4861 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     4921 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     4981 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     5041 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     5101 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     5161 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     5221 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     5281 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     5341 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     5401 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     5461 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     5521 gtatttacat taaatggcca tagttg
//
