LOCUS       sequence-168589-        5478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP4K3, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP4K3_p.R753Q
FEATURES             Location/Qualifiers
     source          1..5478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      24..612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     513..532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     766..783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(942..969)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1061..1147)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1196..1218
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1210..1227
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1211..1227
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1243..1342
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4031..4130)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4147..4166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4148..4166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4171..4187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4171..4187)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4618..5409
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
       61 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      781 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      841 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      901 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
      961 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     1021 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     1081 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     1141 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     1201 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
     1261 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
     1321 actttgtaca aaaaagttgg caccatgaac cccggcttcg atttgtcccg ccggaacccg
     1381 caggaggact tcgagctgat tcagcgcatc ggcagcggca cctacggcga cgtctacaag
     1441 gcacggaatg ttaacactgg tgaattagca gcaattaaag taataaaatt ggaaccagga
     1501 gaagactttg cagttgtgca gcaagaaatt atcatgatga aagactgtaa acacccaaat
     1561 attgttgctt attttggaag ctatctcagg cgagataagc tttggatttg catggagttt
     1621 tgtggaggtg gttctttaca ggatatttat cacgtaactg gacctctgtc agaactgcaa
     1681 attgcatatg ttagcagaga aacactgcag ggattatatt atcttcacag taaaggaaaa
     1741 atgcacagag atataaaggg agctaacatt ctattaacgg ataatggtca tgtgaaattg
     1801 gctgattttg gagtatctgc acagataaca gctacaattg ccaaacggaa gtctttcatt
     1861 ggcacaccat attggatggc tccagaagtt gcagctgttg agaggaaggg gggttacaat
     1921 caactctgtg atctctgggc agtgggaatc actgccatag aacttgcaga gcttcagcct
     1981 cctatgtttg acttacaccc aatgagagca ttatttctaa tgacaaaaag caattttcag
     2041 cctcctaaac taaaggataa aatgaaatgg tcaaatagtt ttcatcactt tgtgaaaatg
     2101 gcacttacca aaaatccgaa aaaaagacct actgctgaaa aattattaca gcatcctttt
     2161 gtaacacaac atttgacacg gtctttggca atcgagctgt tggataaagt aaataatcca
     2221 gatcattcca cttaccatga tttcgatgat gatgatcctg agcctcttgt tgctgtacca
     2281 catagaattc actcaacaag tagaaacgtg agagaagaaa aaacacgctc agagataacc
     2341 tttggccaag tgaaatttga tccaccctta agaaaggaga cagaaccaca tcatgaactt
     2401 cccgacagtg atggtttttt ggacagttca gaagaaatat actacactgc aagatctaat
     2461 ctggatctgc aactggaata tggacaagga caccaaggtg gttacttttt aggtgcaaac
     2521 aagagtcttc tcaagtctgt tgaagaagaa ttgcatcagc gaggacacgt cgcacattta
     2581 gaagatgatg aaggagatga tgatgaatct aaacactcaa ctctgaaagc aaaaattcca
     2641 cctcctttgc caccaaagcc taagtctatc ttcataccac aggaaatgca ttctactgag
     2701 gatgaaaatc aaggaacaat caagagatgt cccatgtcag ggagcccagc aaagccatcc
     2761 caagttccac ctagaccacc acctcccaga ttacccccac acaaacctgt tgccttagga
     2821 aatggaatga gctccttcca gttaaatggt gaacgagatg gctcattatg tcaacaacag
     2881 aatgaacata gaggcacaaa cctttcaaga aaagaaaaga aagatgtacc aaagcctatt
     2941 agtaatggtc ttcctccaac acctaaagtg catatgggtg catgtttttc aaaagttttt
     3001 aatgggtgtc ccttgaaaat tcactgtgca tcatcatgga taaacccaga tacaagagat
     3061 cagtacttga tatttggtgc cgaagaaggg atttataccc tcaatcttaa tgaacttcat
     3121 gaaacatcaa tggaacagct attccctcga aggtgtacat ggttgtatgt aatgaacaat
     3181 tgcttgctat caatatctgg taaagcttct cagctttatt cccataattt accagggctt
     3241 tttgattatg caagacaaat gcaaaagtta cctgttgcta ttccagcaca caaactccct
     3301 gacagaatac tgccaaggaa attttctgta tcagcaaaaa tccctgaaac caaatggtgc
     3361 cagaagtgtt gtgttgtaag aaatccttac acgggccata aatacctatg tggagcactt
     3421 cagactagca ttgttctatt agaatgggtt gaaccaatgc agaaatttat gttaattaag
     3481 cacatagatt ttcctatacc atgtccactt agaatgtttg aaatgctggt agttcctgaa
     3541 caggagtacc ctttagtttg tgttggtgtc agtagaggta gagacttcaa ccaagtggtt
     3601 caatttgaga cggtcaatcc aaattctacc tcttcatggt ttacagaatc agatacccca
     3661 cagacaaatg ttactcatgt aacccaactg gagagagata ccatccttgt atgcttggac
     3721 tgttgtataa aaatagtaaa tctccaagga agattaaaat ctagcaggaa attgtcatca
     3781 gaactcacct ttgatttcca gattgaatca atagtgtgcc tacaagacag tgtgctagct
     3841 ttctggaaac atggaatgca aggtagaagt tttagatcta atgaggtaac acaagaaatt
     3901 tcagatagca caagaatttt caggctgctt ggatctgaca gggtcgtggt tttggaaagt
     3961 aggccaactg ataaccccac agcaaatagc aatttgtaca tcctggcggg tcatgaaaac
     4021 agttactgat tgccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     4081 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     4141 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     4201 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     4261 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     4321 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     4381 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     4441 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     4501 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     4561 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     4621 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     4681 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     4741 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     4801 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     4861 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     4921 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     4981 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     5041 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     5101 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     5161 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     5221 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     5281 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     5341 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     5401 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     5461 cactgagcgt cagacccc
//
