LOCUS       sequence-168594-        5446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.H36Y
FEATURES             Location/Qualifiers
     source          1..5446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     515..534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     768..785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(944..971)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1063..1149)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1198..1220
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1212..1229
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1213..1229
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1245..1344
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1347..3692
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIYSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3694..3793)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3811..3829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4001..4100)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4118..4136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4141..4157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4141..4157)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4588..5379
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
       61 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      121 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      181 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      241 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      301 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      361 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      421 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      481 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      541 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
      601 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
      661 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      721 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      781 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      841 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      901 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
      961 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     1021 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     1081 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     1141 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     1201 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat
     1261 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatga
     1321 caactttgta caaaaaagtt ggcaccatgg ctactcaagc tgatttgatg gagttggaca
     1381 tggccatgga accagacaga aaagcggctg ttagtcactg gcagcaacag tcttacctgg
     1441 actctggaat ctattctggt gccactacca cagctccttc tctgagtggt aaaggcaatc
     1501 ctgaggaaga ggatgtggat acctcccaag tcctgtatga gtgggaacag ggattttctc
     1561 agtccttcac tcaagaacaa gtagctgata ttgatggaca gtatgcaatg actcgagctc
     1621 agagggtacg agctgctatg ttccctgaga cattagatga gggcatgcag atcccatcta
     1681 cacagtttga tgctgctcat cccactaatg tccagcgttt ggctgaacca tcacagatgc
     1741 tgaaacatgc agttgtaaac ttgattaact atcaagatga tgcagaactt gccacacgtg
     1801 caatccctga actgacaaaa ctgctaaatg acgaggacca ggtggtggtt aataaggctg
     1861 cagttatggt ccatcagctt tctaaaaagg aagcttccag acacgctatc atgcgttctc
     1921 ctcagatggt gtctgctatt gtacgtacca tgcagaatac aaatgatgta gaaacagctc
     1981 gttgtaccgc tgggaccttg cataaccttt cccatcatcg tgagggctta ctggccatct
     2041 ttaagtctgg aggcattcct gccctggtga aaatgcttgg ttcaccagtg gattctgtgt
     2101 tgttttatgc cattacaact ctccacaacc ttttattaca tcaagaagga gctaaaatgg
     2161 cagtgcgttt agctggtggg ctgcagaaaa tggttgcctt gctcaacaaa acaaatgtta
     2221 aattcttggc tattacgaca gactgccttc aaattttagc ttatggcaac caagaaagca
     2281 agctcatcat actggctagt ggtggacccc aagctttagt aaatataatg aggacctata
     2341 cttacgaaaa actactgtgg accacaagca gagtgctgaa ggtgctatct gtctgctcta
     2401 gtaataagcc ggctattgta gaagctggtg gaatgcaagc tttaggactt cacctgacag
     2461 atccaagtca acgtcttgtt cagaactgtc tttggactct caggaatctt tcagatgctg
     2521 caactaaaca ggaagggatg gaaggtctcc ttgggactct tgttcagctt ctgggttcag
     2581 atgatataaa tgtggtcacc tgtgcagctg gaattctttc taacctcact tgcaataatt
     2641 ataagaacaa gatgatggtc tgccaagtgg gtggtataga ggctcttgtg cgtactgtcc
     2701 ttcgggctgg tgacagggaa gacatcactg agcctgccat ctgtgctctt cgtcatctga
     2761 ccagccgaca ccaagaagca gagatggccc agaatgcagt tcgccttcac tatggactac
     2821 cagttgtggt taagctctta cacccaccat cccactggcc tctgataaag gctactgttg
     2881 gattgattcg aaatcttgcc ctttgtcccg caaatcatgc acctttgcgt gagcagggtg
     2941 ccattccacg actagttcag ttgcttgttc gtgcacatca ggatacccag cgccgtacgt
     3001 ccatgggtgg gacacagcag caatttgtgg agggggtccg catggaagaa atagttgaag
     3061 gttgtaccgg agcccttcac atcctagctc gggatgttca caaccgaatt gttatcagag
     3121 gactaaatac cattccattg tttgtgcagc tgctttattc tcccattgaa aacatccaaa
     3181 gagtagctgc aggggtcctc tgtgaacttg ctcaggacaa ggaagctgca gaagctattg
     3241 aagctgaggg agccacagct cctctgacag agttacttca ctctaggaat gaaggtgtgg
     3301 cgacatatgc agctgctgtt ttgttccgaa tgtctgagga caagccacaa gattacaaga
     3361 aacggctttc agttgagctg accagctctc tcttcagaac agagccaatg gcttggaatg
     3421 agactgctga tcttggactt gatattggtg cccagggaga accccttgga tatcgccagg
     3481 atgatcctag ctatcgttct tttcactctg gtggatatgg ccaggatgcc ttgggtatgg
     3541 accccatgat ggaacatgag atgggtggcc accaccctgg tgctgactat ccagttgatg
     3601 ggctgccaga tctggggcat gcccaggacc tcatggatgg gctgcctcca ggtgacagca
     3661 atcagctggc ctggtttgat actgacctgt aattgccaac tttcttgtac aaagttggca
     3721 ttataagaaa gcattgctta tcaatttgtt tcaacgtaca ggtcactatc agtcaaaata
     3781 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atgccgaatc
     3841 cagtggagca gcgttacatg gagctcttag ccttacgcga cgaatacata aagcggcttg
     3901 aggaactgca gctcgccaac tctgccaagc tttctgatcc cccaacttca ccttccagtc
     3961 gacagcaatm wgatggcctg gtttgatact gacctgtaat tgccaacttt cttgtacaaa
     4021 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     4081 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     4141 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     4201 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     4261 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     4321 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     4381 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     4441 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     4501 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     4561 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     4621 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     4681 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     4741 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     4801 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     4861 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     4921 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     4981 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     5041 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     5101 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     5161 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     5221 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     5281 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     5341 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     5401 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagacc
//
