LOCUS       sequence-168601-        4119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing HTR3B, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_HTR3B_p.W95C
FEATURES             Location/Qualifiers
     source          1..4119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(934..961)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1053..1139)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1188..1210
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1202..1219
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1203..1219
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1235..1334
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2664..2763)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2780..2799)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2781..2799)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2804..2820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2804..2820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3251..4042
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      841 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
      901 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      961 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
     1021 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
     1081 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
     1141 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
     1201 ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat
     1261 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
     1321 caaaaaagtt ggcaccatgt tgtcaagtgt aatggctccc ctgtgggcct gcatcctggt
     1381 ggctgcagga attctagcca cagatacaca tcatccccag gattctgctc tgtatcatct
     1441 cagcaagcag ctattacaga aatatcataa agaagtgaga cctgtttaca actggaccaa
     1501 ggccaccaca gtctacctgg acctgttcgt ccatgctata ttggatgtgg atgcagagaa
     1561 tcaaatatta aagacaagtg tatggtacca agaggtctgg aatgatgaat ttttatcctg
     1621 taactccagc atgtttgatg agattagaga gatctcccta cctctaagtg ccatctgggc
     1681 ccccgatatc atcatcaatg agtttgtgga cattgaaaga taccctgacc ttccctatgt
     1741 ttatgtgaac tcatctggga ccattgagaa ctataagccc atccaggtgg tctctgcgtg
     1801 cagtttagag acatatgctt ttccatttga tgtccagaat tgcagcctga ccttcaagag
     1861 cattctgcat acagtggaag acgtagacct ggcctttctg aggagcccag aagacattca
     1921 gcatgacaaa aaggcgtttt tgaatgacag tgagtgggaa cttctatctg tgtcctccac
     1981 atacagcatc ctgcagagca gcgctggagg atttgcacag attcagttta atgtggtgat
     2041 gcgcaggcac cccctggtct atgtcgtgag tctgctgatt cctagcatct ttctcatgct
     2101 ggtggacctg gggagcttct acctgccacc caactgccga gccaggattg tgttcaagac
     2161 cagtgtgctg gtgggctaca ccgtcttcag ggtcaacatg tccaaccagg tgccacggag
     2221 tgtagggagc acccctctga ttgggcactt cttcaccatc tgcatggcct tcttggttct
     2281 cagcttagct aagtccatcg tgttggtcaa attcctccat gatgagcagc gtggtggaca
     2341 ggagcagccc ttcttgtgcc ttcgagggga caccgatgct gacaggccta gagtggaacc
     2401 cagggcccaa cgtgctgtgg taacagagtc ctcgctgtat ggagagcacc tggcccagcc
     2461 aggaaccctg aaggaagtct ggtcgcagct tcaatctatc agcaactacc tccaaactca
     2521 ggaccagaca gaccaacagg aggcagagtg gctggtcctc ctgtcccgct ttgaccgact
     2581 gctcttccaa agctaccttt tcatgctggg gatctacacc atcactctgt gctccctctg
     2641 ggcactgtgg ggcggcgtgt gattgccaac tttcttgtac aaagttggca ttataagaaa
     2701 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2761 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2821 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2881 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     2941 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3001 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3061 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3121 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3181 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3241 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3301 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3361 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3421 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3481 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3541 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     3601 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     3661 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     3721 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     3781 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     3841 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     3901 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     3961 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4021 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4081 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaa
//
