LOCUS       sequence-168603-        5138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.G34R
FEATURES             Location/Qualifiers
     source          1..5138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     526..545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     779..796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(955..982)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1074..1160)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1209..1231
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1223..1240
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1224..1240
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1256..1355
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1358..3703
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSRIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(3705..3804)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3821..3840)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3822..3840)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3845..3861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3845..3861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4291..5082
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
       61 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      841 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      901 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
      961 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     1021 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     1081 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     1141 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     1201 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
     1261 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
     1321 ttttataatg acaactttgt acaaaaaagt tggcaccatg gctactcaag ctgatttgat
     1381 ggagttggac atggccatgg aaccagacag aaaagcggct gttagtcact ggcagcaaca
     1441 gtcttacctg gactctagaa tccattctgg tgccactacc acagctcctt ctctgagtgg
     1501 taaaggcaat cctgaggaag aggatgtgga tacctcccaa gtcctgtatg agtgggaaca
     1561 gggattttct cagtccttca ctcaagaaca agtagctgat attgatggac agtatgcaat
     1621 gactcgagct cagagggtac gagctgctat gttccctgag acattagatg agggcatgca
     1681 gatcccatct acacagtttg atgctgctca tcccactaat gtccagcgtt tggctgaacc
     1741 atcacagatg ctgaaacatg cagttgtaaa cttgattaac tatcaagatg atgcagaact
     1801 tgccacacgt gcaatccctg aactgacaaa actgctaaat gacgaggacc aggtggtggt
     1861 taataaggct gcagttatgg tccatcagct ttctaaaaag gaagcttcca gacacgctat
     1921 catgcgttct cctcagatgg tgtctgctat tgtacgtacc atgcagaata caaatgatgt
     1981 agaaacagct cgttgtaccg ctgggacctt gcataacctt tcccatcatc gtgagggctt
     2041 actggccatc tttaagtctg gaggcattcc tgccctggtg aaaatgcttg gttcaccagt
     2101 ggattctgtg ttgttttatg ccattacaac tctccacaac cttttattac atcaagaagg
     2161 agctaaaatg gcagtgcgtt tagctggtgg gctgcagaaa atggttgcct tgctcaacaa
     2221 aacaaatgtt aaattcttgg ctattacgac agactgcctt caaattttag cttatggcaa
     2281 ccaagaaagc aagctcatca tactggctag tggtggaccc caagctttag taaatataat
     2341 gaggacctat acttacgaaa aactactgtg gaccacaagc agagtgctga aggtgctatc
     2401 tgtctgctct agtaataagc cggctattgt agaagctggt ggaatgcaag ctttaggact
     2461 tcacctgaca gatccaagtc aacgtcttgt tcagaactgt ctttggactc tcaggaatct
     2521 ttcagatgct gcaactaaac aggaagggat ggaaggtctc cttgggactc ttgttcagct
     2581 tctgggttca gatgatataa atgtggtcac ctgtgcagct ggaattcttt ctaacctcac
     2641 ttgcaataat tataagaaca agatgatggt ctgccaagtg ggtggtatag aggctcttgt
     2701 gcgtactgtc cttcgggctg gtgacaggga agacatcact gagcctgcca tctgtgctct
     2761 tcgtcatctg accagccgac accaagaagc agagatggcc cagaatgcag ttcgccttca
     2821 ctatggacta ccagttgtgg ttaagctctt acacccacca tcccactggc ctctgataaa
     2881 ggctactgtt ggattgattc gaaatcttgc cctttgtccc gcaaatcatg cacctttgcg
     2941 tgagcagggt gccattccac gactagttca gttgcttgtt cgtgcacatc aggataccca
     3001 gcgccgtacg tccatgggtg ggacacagca gcaatttgtg gagggggtcc gcatggaaga
     3061 aatagttgaa ggttgtaccg gagcccttca catcctagct cgggatgttc acaaccgaat
     3121 tgttatcaga ggactaaata ccattccatt gtttgtgcag ctgctttatt ctcccattga
     3181 aaacatccaa agagtagctg caggggtcct ctgtgaactt gctcaggaca aggaagctgc
     3241 agaagctatt gaagctgagg gagccacagc tcctctgaca gagttacttc actctaggaa
     3301 tgaaggtgtg gcgacatatg cagctgctgt tttgttccga atgtctgagg acaagccaca
     3361 agattacaag aaacggcttt cagttgagct gaccagctct ctcttcagaa cagagccaat
     3421 ggcttggaat gagactgctg atcttggact tgatattggt gcccagggag aaccccttgg
     3481 atatcgccag gatgatccta gctatcgttc ttttcactct ggtggatatg gccaggatgc
     3541 cttgggtatg gaccccatga tggaacatga gatgggtggc caccaccctg gtgctgacta
     3601 tccagttgat gggctgccag atctggggca tgcccaggac ctcatggatg ggctgcctcc
     3661 aggtgacagc aatcagctgg cctggtttga tactgacctg taattgccaa ctttcttgta
     3721 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     3781 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     3841 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     3901 tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc
     3961 gactcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     4021 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     4081 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     4141 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     4201 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     4261 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
     4321 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     4381 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     4441 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     4501 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     4561 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     4621 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     4681 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     4741 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     4801 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     4861 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     4921 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     4981 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     5041 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     5101 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactg
//
