LOCUS       sequence-168604-        4914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FBXW7 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FBXW7_p.A502V
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2793..2892)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2909..2928)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2910..2928)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2933..2949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2933..2949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3380..4171
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4264..4852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4753..4772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaatcagg aactgctctc tgtgggcagc aaaagacgac gaactggagg
      721 ctctctgaga ggtaaccctt cctcaagcca ggtagatgaa gaacagatga atcgtgtggt
      781 agaggaggaa cagcaacagc aactcagaca acaagaggag gagcacactg caaggaatgg
      841 tgaagttgtt ggagtagaac ctagacctgg aggccaaaat gattcccagc aaggacagtt
      901 ggaagaaaac aataatagat ttatttcggt agatgaggac tcctcaggaa accaagaaga
      961 acaagaggaa gatgaagaac atgctggtga acaagatgag gaggatgagg aggaggagga
     1021 gatggaccag gagagtgacg attttgatca gtctgatgat agtagcagag aagatgaaca
     1081 tacacatact aacagtgtca cgaactccag tagtattgtg gacctgcccg ttcaccaact
     1141 ctcctcccca ttctatacaa aaacaacaaa aatgaaaaga aagttggacc atggttctga
     1201 ggtccgctct ttttctttgg gaaagaaacc atgcaaagtc tcagaatata caagtaccac
     1261 tgggcttgta ccatgttcag caacaccaac aacttttggg gacctcagag cagccaatgg
     1321 ccaagggcaa caacgacgcc gaattacatc tgtccagcca cctacaggcc tccaggaatg
     1381 gctaaaaatg tttcagagct ggagtggacc agagaaattg cttgctttag atgaactcat
     1441 tgatagttgt gaaccaacac aagtaaaaca tatgatgcaa gtgatagaac cccagtttca
     1501 acgagacttc atttcattgc tccctaaaga gttggcactc tatgtgcttt cattcctgga
     1561 acccaaagac ctgctacaag cagctcagac atgtcgctac tggagaattt tggctgaaga
     1621 caaccttctc tggagagaga aatgcaaaga agaggggatt gatgaaccat tgcacatcaa
     1681 gagaagaaaa gtaataaaac caggtttcat acacagtcca tggaaaagtg catacatcag
     1741 acagcacaga attgatacta actggaggcg aggagaactc aaatctccta aggtgctgaa
     1801 aggacatgat gatcatgtga tcacatgctt acagttttgt ggtaaccgaa tagttagtgg
     1861 ttctgatgac aacactttaa aagtttggtc agcagtcaca ggcaaatgtc tgagaacatt
     1921 agtgggacat acaggtggag tatggtcatc acaaatgaga gacaacatca tcattagtgg
     1981 atctacagat cggacactca aagtgtggaa tgcagagact ggagaatgta tacacacctt
     2041 atatgggcat acttccactg tgcgttgtat gcatcttcat gaaaaaagag ttgttagcgg
     2101 ttctcgagat gccactctta gggtttggga tattgagaca ggccagtgtt tacatgtttt
     2161 gatgggtcat gttgtagcag tccgctgtgt tcaatatgat ggcaggaggg ttgttagtgg
     2221 agcatatgat tttatggtaa aggtgtggga tccagagact gaaacctgtc tacacacgtt
     2281 gcaggggcat actaatagag tctattcatt acagtttgat ggtatccatg tggtgagtgg
     2341 atctcttgat acatcaatcc gtgtttggga tgtggagaca gggaattgca ttcacacgtt
     2401 aacagggcac cagtcgttaa caagtggaat ggaactcaaa gacaatattc ttgtctctgg
     2461 gaatgcagat tctacagtta aaatctggga tatcaaaaca ggacagtgtt tacaaacatt
     2521 gcaaggtccc aacaagcatc agagtgctgt gacctgttta cagttcaaca agaactttgt
     2581 aattaccagc tcagatgatg gaactgtaaa actatgggac ttgaaaacgg gtgaatttat
     2641 tcgaaaccta gtcacattgg agagtggggg gagtggggga gttgtgtggc ggatcagagc
     2701 ctcaaacaca aagctggtgt gtgcagttgg gagtcggaat gggactgaag aaaccaagct
     2761 gctggtgctg gactttgatg tggacatgaa gttgccaact ttcttgtaca aagttggcat
     2821 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2881 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2941 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3001 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     3061 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     3121 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     3181 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3241 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3301 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3361 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3421 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3481 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3541 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3601 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3661 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3721 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3781 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3841 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3901 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3961 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     4021 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     4081 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     4141 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     4201 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
