LOCUS       sequence-168605-        4512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAM200A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FAM200A_p.S481N
FEATURES             Location/Qualifiers
     source          1..4512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2391..2490)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2507..2526)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2508..2526)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2531..2547)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2531..2547)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2978..3769
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3862..4450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4351..4370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg actcctgaat caagggatac tacagatttg tctccagggg gtacccagga
      721 gatggaaggc atcgtgatag tgaaggtgga ggaggaagat gaagaagacc attttcaaaa
      781 ggaaagaaac aaagtagagt catcgccaca agttctcagt cgctctacaa ctatgaatga
      841 gagagcctta ttgtcatcgt atttagttgc atatagagtg gcaaaagaga aaatggctca
      901 cacagcggct gaaaaaatta tccttccagc atgtatggac atggtacgga caatttttga
      961 tgacaaatca gctgataaac taagaactat acctcttagt gataatacaa tatctcgtcg
     1021 aatctgtacg attgcaaaac atttggaagc aatgcttatt acacggctgc agtccggtat
     1081 agactttgca atccaactcg atgagagcac tgatattgca agttgtccca cactcttggt
     1141 ttatgtcaga tatgtgtggc aagatgattt tgtagaggat ctcttatgtt gtttaaattt
     1201 aaattcacat ataactggat tagatttatt tactgaatta gaaaactgcc ttcttggtca
     1261 gtataaatta aactggaaac attgtaaagg aatttcaagt gatggaacag caaatatgac
     1321 cggaaaacac agcagactta ctgaaaaatt gttagaagca acccacaaca atgctgtttg
     1381 gaatcactgt tttattcatc gagaagcttt ggtatccaaa gaaatttcac caagtctgat
     1441 ggatgtattg aaaaatgcag tgaaaactgt taattttatt aaaggaagct cactgaatag
     1501 ccgacttctc gaaatatttt gttcagagat tggagtgaac cacacccact tattgtttca
     1561 tacagaagtt cgttggcttt ctcaaggaaa agtattgagc agagtatatg aactcaggaa
     1621 cgagatttac atttttctcg ttgaaaagca atctcatttg gcaaatattt ttgaagacga
     1681 catttgggta acaaaattgg catatttaag tgatattttt ggcattctta atgaattaag
     1741 cctgaaaatg caggggaaaa acaatgatat atttcagtat cttgaacata ttctaggatt
     1801 ccaaaagacg ttattattgt ggcaagcaag acttaaaagt aaccgcccta gctactatat
     1861 gtttccaaca ttattgcaac acatcgaaga gaacattatt aatgaagact gcttaaaaga
     1921 aataaaatta gagatattgt tgcatctcac ttctttgtct caaactttta attattactt
     1981 tccggaagag aaatttgaat cattaaagga aaatatttgg atgaaagatc catttgcttt
     2041 tcaaaaccca gaatcaataa ttgagttaaa cttggagcct gaagaagaga atgaattatt
     2101 gcagctcaat tcatcattca cactaaagaa ttattataag atattaagtt tatcagcatt
     2161 ttggattaag attaaagatg actttccact gctaagtagg aagagtatat tgctgttact
     2221 accattcaca actacatatt tgtgtgaact aggattttca atcttgacac ggttaaaaac
     2281 aaagaagaga aataggctca atagtgcacc agatatgcgg gtagcattat cttcatgtgt
     2341 tcctgactgg aaggaactta tgaacagaca agcacaccca tcacattgat tgccaacttt
     2401 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2461 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2521 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2581 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     2641 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     2701 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     2761 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2821 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     2881 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     2941 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     3001 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     3061 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     3121 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     3181 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     3241 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     3301 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     3361 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     3421 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     3481 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     3541 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     3601 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     3661 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     3721 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     3781 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3841 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3901 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3961 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4021 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4081 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4141 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4201 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4261 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4321 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4381 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4441 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4501 tgctcacatg tt
//
