LOCUS       sequence-168606-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTEN, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTEN_p.K6N
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     173..192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     426..443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(602..629)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(721..807)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     856..878
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     870..887
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     871..887
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    903..1002
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2218..2317)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2334..2353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2335..2353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2358..2374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2358..2374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2805..3596
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3689..4005,1..272)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
       61 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
      481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      541 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
      601 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
      661 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
      721 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
      781 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      841 tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      901 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      961 aatgcttttt tataatgcca actttgtaca aaaaagttgg caccatgaca gccatcatca
     1021 atgagatcgt tagcagaaac aaaaggagat atcaagagga tggattcgac ttagacttga
     1081 cctatattta tccaaacatt attgctatgg gatttcctgc agaaagactt gaaggcgtat
     1141 acaggaacaa tattgatgat gtagtaaggt ttttggattc aaagcataaa aaccattaca
     1201 agatatacaa tctttgtgct gaaagacatt atgacaccgc caaatttaat tgcagagttg
     1261 cacaatatcc ttttgaagac cataacccac cacagctaga acttatcaaa cccttttgtg
     1321 aagatcttga ccaatggcta agtgaagatg acaatcatgt tgcagcaatt cactgtaaag
     1381 ctggaaaggg acgaactggt gtaatgatat gtgcatattt attacatcgg ggcaaatttt
     1441 taaaggcaca agaggcccta gatttctatg gggaagtaag gaccagagac aaaaagggag
     1501 taactattcc cagtcagagg cgctatgtgt attattatag ctacctgtta aagaatcatc
     1561 tggattatag accagtggca ctgttgtttc acaagatgat gtttgaaact attccaatgt
     1621 tcagtggcgg aacttgcaat cctcagtttg tggtctgcca gctaaaggtg aagatatatt
     1681 cctccaattc aggacccaca cgacgggaag acaagttcat gtactttgag ttccctcagc
     1741 cgttacctgt gtgtggtgat atcaaagtag agttcttcca caaacagaac aagatgctaa
     1801 aaaaggacaa aatgtttcac ttttgggtaa atacattctt cataccagga ccagaggaaa
     1861 cctcagaaaa agtagaaaat ggaagtctat gtgatcaaga aatcgatagc atttgcagta
     1921 tagagcgtgc agataatgac aaggaatatc tagtacttac tttaacaaaa aatgatcttg
     1981 acaaagcaaa taaagacaaa gccaaccgat acttttctcc aaattttaag gtgaagctgt
     2041 acttcacaaa aacagtagag gagccgtcaa atccagaggc tagcagttca acttctgtaa
     2101 caccagatgt tagtgacaat gaacctgatc attatagata ttctgacacc actgactctg
     2161 atccagagaa tgaacctttt gatgaagatc agcatacaca aattacaaaa gtctgattgc
     2221 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2281 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2341 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2401 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2461 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2521 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2581 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2641 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2701 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2761 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2821 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2881 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2941 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3001 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3061 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3121 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3181 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3241 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3301 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3361 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3421 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3481 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3541 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3601 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggct
//
