LOCUS       sequence-168608-        4695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing POT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_POT1_p.G76V
FEATURES             Location/Qualifiers
     source          1..4695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2574..2673)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2690..2709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2691..2709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2714..2730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2714..2730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3161..3952
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4045..4633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4534..4553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa
      661 agttggcacc atgtctttgg ttccagcaac aaattatata tatacacccc tgaatcaact
      721 taagggtggt acaattgtca atgtctatgg tgttgtgaag ttctttaagc ccccatatct
      781 aagcaaagga actgattatt gctcagttgt aactattgtg gaccagacaa atgtaaaact
      841 aacttgcctg ctctttagtg gaaactatga agcccttcca ataatttata aaaatgtaga
      901 tattgttcgc tttcacaggc tgaagattca agtatataaa aaggagactc agggtatcac
      961 cagctctggc tttgcatctt tgacgtttga gggaactttg ggagccccta tcatacctcg
     1021 cacttcaagc aagtatttta acttcactac tgaggaccac aaaatggtag aagccttacg
     1081 tgtttgggca tctactcata tgtcaccgtc ttggacatta ctaaaattgt gtgatgttca
     1141 gccaatgcag tattttgacc tgacttgtca gctcttgggc aaagcagaag tggacggagc
     1201 atcatttctt ctaaaggtat gggatggcac caggacacca tttccatctt ggagagtctt
     1261 aatacaagac cttgttcttg aaggtgattt aagtcacatc catcggctac aaaatctgac
     1321 aatagacatt ttagtctacg ataaccatgt tcatgtggca agatctctga aggttggaag
     1381 ctttcttaga atctatagcc ttcataccaa acttcaatca atgaattcag agaatcagac
     1441 aatgttaagt ttagagtttc atcttcatgg aggtaccagt tacggtcggg gaatcagggt
     1501 cttgccagaa agtaactctg atgtggatca actgaaaaag gatttagaat ctgcaaattt
     1561 gacagccaat cagcattcag atgttatctg tcaatcagaa cctgacgaca gctttccaag
     1621 ctctggatca gtatcattat acgaggtaga aagatgtcaa cagctatctg ctacaatact
     1681 tacagatcat cagtatttgg agaggacacc actatgtgcc attttgaaac aaaaagctcc
     1741 tcaacaatac cgcatccgag caaaattgag gtcatataag cccagaagac tatttcagtc
     1801 tgttaaactt cattgcccta aatgtcattt gctgcaagaa gttccacatg agggcgattt
     1861 ggatataatt tttcaggatg gtgcaactaa aaccccagat gtcaagctac aaaatacatc
     1921 attatatgat tcaaaaatct ggaccactaa aaatcaaaaa ggacgaaaag tagcagttca
     1981 ttttgtgaaa aataatggta ttctcccgct ttcaaatgaa tgtctacttt tgatagaagg
     2041 aggtacactc agtgaaattt gcaaactctc gaacaagttt aatagtgtaa ttcctgtgag
     2101 atctggccac gaagacctgg aacttttgga cctttcagca ccatttctta tacaaggaac
     2161 aatacatcac tatggatgta aacagtgttc tagtttgaga tccatacaaa atctaaattc
     2221 cctggttgat aaaacatcgt ggattccttc ttctgtggca gaagcactgg gtattgtacc
     2281 cctccaatat gtgtttgtta tgacctttac acttgatgat ggaacaggag tactagaagc
     2341 ctatctcatg gattctgaca aattcttcca gattccagca tcagaagttc tgatggatga
     2401 tgaccttcag aaaagtgtgg atatgatcat ggatatgttt tgtcctccag gaataaaaat
     2461 tgatgcatat ccgtggttgg aatgcttcat caagtcatac aatgtcacaa atggaacaga
     2521 taatcaaatt tgctatcaga tttttgacac cacagttgca gaagatgtaa tctgcccaac
     2581 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2641 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2701 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2761 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     2821 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
     2881 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     2941 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     3001 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     3061 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     3121 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc
     3181 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     3241 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     3301 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3361 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     3421 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     3481 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3541 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3601 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3661 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3721 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3781 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3841 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3901 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     3961 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     4021 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4081 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4141 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4201 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4261 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4321 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4381 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4441 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4501 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4561 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4621 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4681 ttttgctcac atgtt
//
