LOCUS       sequence-168610-        4185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MEF2C, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MEF2C_p.T9M
FEATURES             Location/Qualifiers
     source          1..4185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      19..607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     508..527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     761..778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(937..964)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1056..1142)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1191..1213
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1205..1222
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1206..1222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1238..1337
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2733..2832)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2849..2868)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2850..2868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2873..2889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2873..2889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3320..4111
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
       61 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
      901 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
      961 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     1021 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     1081 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     1141 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
     1201 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
     1261 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
     1321 gtacaaaaaa gttggcacca tggggagaaa aaagattcag attatgagga ttatggatga
     1381 acgtaacaga caggtgacat ttacaaagag gaaatttggg ttgatgaaga aggcttatga
     1441 gctgagcgtg ctgtgtgact gtgagattgc gctgatcatc ttcaacagca ccaacaagct
     1501 gttccagtat gccagcaccg acatggacaa agtgcttctc aagtacacgg agtacaacga
     1561 gccgcatgag agccggacaa actcagacat cgtggaggca ttgaacaaga aagaaaacaa
     1621 aggctgtgaa agccccgatc ccgactcctc ttatgcactc accccacgca ctgaagaaaa
     1681 atacaaaaaa attaatgaag aatttgataa tatgatcaag agtcataaaa ttcctgctgt
     1741 tccacctccc aacttcgaga tgccagtctc catcccagtg tccagccaca acagtttggt
     1801 gtacagcaac cctgtcagct cactgggaaa ccccaaccta ttgccactgg ctcacccttc
     1861 tctgcagagg aatagtatgt ctcctggtgt aacacatcga cctccaagtg caggtaacac
     1921 aggtggtctg atgggtggag acctcacgtc tggtgcaggc accagtgcag ggaacgggta
     1981 tggcaatccc cgaaactcac caggtctgct ggtctcacct ggtaacttga acaagaatat
     2041 gcaagcaaaa tctcctcccc caatgaattt aggaatgaat aaccgtaaac cagatctccg
     2101 agttcttatt ccaccaggca gcaagaatac gatgccatca gtgaatcaaa ggataaataa
     2161 ctcccagtcg gctcagtcat tggctacccc agtggtttcc gtagcaactc ctactttacc
     2221 aggacaagga atgggaggat atccatcagc catttcaaca acatatggta ccgagtactc
     2281 tctgagtagt gcagacctgt catctctgtc tgggtttaac accgccagcg ctcttcacct
     2341 tggttcagta actggctggc aacagcaaca cctacataac atgccaccat ctgccctcag
     2401 tcagttggga gcttgcacta gcactcattt atctcagagt tcaaatctct ccctgccttc
     2461 tactcaaagc ctcaacatca agtcagaacc tgtttctcct cctagagacc gtaccaccac
     2521 cccttcgaga tacccacaac acacgcgcca cgaggcgggg agatctcctg ttgacagctt
     2581 gagcagctgt agcagttcgt acgacgggag cgaccgagag gatcaccgga acgaattcca
     2641 ctcccccatt ggactcacca gaccttcgcc ggacgaaagg gaaagtccct cagtcaagcg
     2701 catgcgactt tctgaaggat gggcaacatg attgccaact ttcttgtaca aagttggcat
     2761 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2821 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2881 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2941 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     3001 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     3061 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     3121 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3181 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3241 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3301 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3361 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3421 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3481 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3541 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3601 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3661 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3721 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3781 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3841 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3901 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3961 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     4021 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     4081 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     4141 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtaga
//
