LOCUS       sequence-168612-        4230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAM196A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FAM196A_p.Q46R
FEATURES             Location/Qualifiers
     source          1..4230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2109..2208)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2225..2244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2226..2244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2249..2265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2249..2265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2696..3487
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAKVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3580..4168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4069..4088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggtcagta aggacaccgg caaatgcata ctcacaacat cggagagtga
      721 agtggaaccc gccgcctgcc tggccctgga gatgaaatac gccctggacc ccaaccggca
      781 gattaaaaaa cggaacaaag ccctgcgggt gcggtttaag gatatctgcg aggcacagaa
      841 tgagcagagg gacacacagc tgtcctcggg ccagctgggg gagaagcggg aggccaagcc
      901 cgtgtcctgc agagcagcct accgcaaata catgacagtg cccgcacgca ggtccatccc
      961 caacgtcacc aagagcacag gcgtgcagac ctcgcccgac cttaagaagt gttaccagac
     1021 gttccctctg gaccgcaaaa aggggaacct caaaagcctc ccagctgcag atccctttaa
     1081 aagccaaaac aatgggtttc taacagatgc gaaagagaag aacgaggctg gacccatgga
     1141 ggaggcccgg ccatgtggcg cggggcgggt gcacaagacc acagccttgg ttttccattc
     1201 caaccaacac atgaacacag tggaccagcc tttgggggtc aactgcacag agccctgtaa
     1261 aagcccggag ccgctcagct atggagaagc tgcgctccaa aactccactc ggcctccatc
     1321 cgaagagccc gattaccagc tgctcgggag ggccaagcag gaccggggga ggccaaactc
     1381 cgaggagccc gctccacctg ccctcaggag ggtgtttaaa acggaggttg ccaccgttta
     1441 cgcacctgcc ctcagtgcca gggcccccga gcctggtttg tcagactctg cagccgccag
     1501 ccagtggtca ctctgcccgg cagatgacga gcggaggaga gccacacatc tcaacgggct
     1561 ccaggcgccc tcggaaactg ccctggcctg ctcacccccg atgcagtgcc tgtcccccga
     1621 atgtagtgag cagccgtcgc agactcacac cccgccgggg ctggggaacc agcctagtcc
     1681 cacagcggtt gctgcaggtg aagaatgcca acgaatcgtg cctcatacgg aagtggtcga
     1741 cctcaaagca caacttcaga tgatggagaa cttgatcagt tcaagccaag aaaccatcaa
     1801 agtgctcttg ggggtcattc aggagttgga aaaaggagag gcccatcggg aagggctttc
     1861 atatcggacg gggcaagaca cagctaattg tgacacatgc aggaacagtg catgtattat
     1921 ctatagtgtg gagctggatt ttaagcagca agaagacaaa ctccagccgg ttctaagaaa
     1981 actccaccct attgaggaaa ctcaggtcat accttcgcct tactctcagg agacttactc
     2041 ctcaactccc aagcaaaaat ccaaaactga atctaaaaag cacggaagat ggaaactctg
     2101 gttccttttg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2161 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2221 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2281 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2341 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2401 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2461 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2521 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2581 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2641 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     2701 ggaagcggtg atcgccaaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2761 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2821 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2881 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2941 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3001 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3061 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3121 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3181 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3241 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3301 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3361 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3421 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3481 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3541 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3601 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3661 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3721 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3781 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3841 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3901 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3961 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4021 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4081 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4141 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4201 ggccttttgc tggccttttg ctcacatgtt
//
