LOCUS       sequence-168614-        4917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FBXW7 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FBXW7_p.R465C
FEATURES             Location/Qualifiers
     source          1..4917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2796..2895)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2912..2931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2913..2931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2936..2952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2936..2952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3383..4174
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4267..4855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4756..4775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgaatcagg aactgctctc tgtgggcagc aaaagacgac gaactggagg
      721 ctctctgaga ggtaaccctt cctcaagcca ggtagatgaa gaacagatga atcgtgtggt
      781 agaggaggaa cagcaacagc aactcagaca acaagaggag gagcacactg caaggaatgg
      841 tgaagttgtt ggagtagaac ctagacctgg aggccaaaat gattcccagc aaggacagtt
      901 ggaagaaaac aataatagat ttatttcggt agatgaggac tcctcaggaa accaagaaga
      961 acaagaggaa gatgaagaac atgctggtga acaagatgag gaggatgagg aggaggagga
     1021 gatggaccag gagagtgacg attttgatca gtctgatgat agtagcagag aagatgaaca
     1081 tacacatact aacagtgtca cgaactccag tagtattgtg gacctgcccg ttcaccaact
     1141 ctcctcccca ttctatacaa aaacaacaaa aatgaaaaga aagttggacc atggttctga
     1201 ggtccgctct ttttctttgg gaaagaaacc atgcaaagtc tcagaatata caagtaccac
     1261 tgggcttgta ccatgttcag caacaccaac aacttttggg gacctcagag cagccaatgg
     1321 ccaagggcaa caacgacgcc gaattacatc tgtccagcca cctacaggcc tccaggaatg
     1381 gctaaaaatg tttcagagct ggagtggacc agagaaattg cttgctttag atgaactcat
     1441 tgatagttgt gaaccaacac aagtaaaaca tatgatgcaa gtgatagaac cccagtttca
     1501 acgagacttc atttcattgc tccctaaaga gttggcactc tatgtgcttt cattcctgga
     1561 acccaaagac ctgctacaag cagctcagac atgtcgctac tggagaattt tggctgaaga
     1621 caaccttctc tggagagaga aatgcaaaga agaggggatt gatgaaccat tgcacatcaa
     1681 gagaagaaaa gtaataaaac caggtttcat acacagtcca tggaaaagtg catacatcag
     1741 acagcacaga attgatacta actggaggcg aggagaactc aaatctccta aggtgctgaa
     1801 aggacatgat gatcatgtga tcacatgctt acagttttgt ggtaaccgaa tagttagtgg
     1861 ttctgatgac aacactttaa aagtttggtc agcagtcaca ggcaaatgtc tgagaacatt
     1921 agtgggacat acaggtggag tatggtcatc acaaatgaga gacaacatca tcattagtgg
     1981 atctacagat cggacactca aagtgtggaa tgcagagact ggagaatgta tacacacctt
     2041 atatgggcat acttccactg tgtgttgtat gcatcttcat gaaaaaagag ttgttagcgg
     2101 ttctcgagat gccactctta gggtttggga tattgagaca ggccagtgtt tacatgtttt
     2161 gatgggtcat gttgcagcag tccgctgtgt tcaatatgat ggcaggaggg ttgttagtgg
     2221 agcatatgat tttatggtaa aggtgtggga tccagagact gaaacctgtc tacacacgtt
     2281 gcaggggcat actaatagag tctattcatt acagtttgat ggtatccatg tggtgagtgg
     2341 atctcttgat acatcaatcc gtgtttggga tgtggagaca gggaattgca ttcacacgtt
     2401 aacagggcac cagtcgttaa caagtggaat ggaactcaaa gacaatattc ttgtctctgg
     2461 gaatgcagat tctacagtta aaatctggga tatcaaaaca ggacagtgtt tacaaacatt
     2521 gcaaggtccc aacaagcatc agagtgctgt gacctgttta cagttcaaca agaactttgt
     2581 aattaccagc tcagatgatg gaactgtaaa actatgggac ttgaaaacgg gtgaatttat
     2641 tcgaaaccta gtcacattgg agagtggggg gagtggggga gttgtgtggc ggatcagagc
     2701 ctcaaacaca aagctggtgt gtgcagttgg gagtcggaat gggactgaag aaaccaagct
     2761 gctggtgctg gactttgatg tggacatgaa gtgattgcca actttcttgt acaaagttgg
     2821 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2881 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2941 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3001 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     3061 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3121 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3181 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3241 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3301 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3361 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3421 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3481 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3541 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3601 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3661 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3721 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3781 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3841 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3901 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     3961 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     4021 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     4081 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4141 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4201 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4381 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4621 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
