LOCUS       sequence-168619-        4590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WBSCR17 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WBSCR17_p.S194R
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2469..2568)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2585..2604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2586..2604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2609..2625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2609..2625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3056..3847
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3940..4528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4429..4448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcttcac tgagaagagt caaagtgctg ttggtgttga acttgatcgc
      721 ggtagccggc ttcgtgctct tcctggccaa gtgccggccc atcgcggtgc gcagcggaga
      781 cgccttccac gagatccggc cgcgcgccga ggtggccaac ctcagcgcgc acagcgccag
      841 ccccatccag gatgcggtcc tgaagcgcct gtcgctgctg gaggacatcg tgtaccggca
      901 gctgaatggc ttatccaaat cccttgggct cattgaaggt tatggtgggc ggggtaaagg
      961 gggccttccg gctactcttt ccccggctga agaagaaaag gctaagggac cccatgagaa
     1021 gtatggctac aattcatacc tcagtgaaaa aatttcactg gaccgttcca ttccggatta
     1081 tcgtcccacc aagtgtaagg agctcaagta ctccaaggac ctgccccaga tatccatcat
     1141 attcatcttc gtgaacgagg ccctgtcggt gatcctgcgg tccgtgcaca gtgccgtcaa
     1201 tcacacgccc acacacctgc tgaaggaaat cattctggtg gatgacaaca gagacgaaga
     1261 ggagctgaag gtccccctag aggagtatgt ccacaaacgc taccccgggc tggtgaaggt
     1321 ggtaagaaat cagaagaggg aaggcctgat ccgcgctcgc attgagggct ggaaggtggc
     1381 taccgggcag gtcactggct tctttgatgc ccacgtggaa ttcaccgctg gctgggctga
     1441 gccggttcta tcccgcatcc aggaaaaccg gaagcgtgtg atcctcccct ccattgacaa
     1501 catcaaacag gacaactttg aggtgcagcg gtacgagaac tcggcccacg ggtacagctg
     1561 ggagctgtgg tgcatgtaca tcagcccccc aaaagactgg tgggacgccg gagacccttc
     1621 tctccccatc aggaccccag ccatgatagg ctgctcgttc gtggtcaaca ggaagttctt
     1681 cggtgaaatt ggtcttctgg atcctggcat ggatgtatac ggaggagaaa atattgaact
     1741 gggaatcaag gtatggctct gtgggggcag catggaggtc cttccttgct cacgggtggc
     1801 ccacattgag cggaagaaga agccatataa tagcaacatt ggcttctaca ccaagaggaa
     1861 tgctcttcgc gttgctgagg tctggatgga cgattacaag tctcatgtgt acatagcgtg
     1921 gaacctgccg ctggagaatc cgggaattga catcggtgat gtctccgaaa gaagagcatt
     1981 aaggaaaagt ttaaagtgta agaatttcca gtggtacctg gaccatgttt acccagaaat
     2041 gagaagatac aataataccg ttgcttacgg ggagcttcgc aacaacaagg caaaagacgt
     2101 ctgcttggac caggggccgc tggagaacca cacagcaata ttgtatccgt gccatggctg
     2161 gggaccacag cttgcccgct acaccaagga aggcttcctg cacttgggtg ccctggggac
     2221 caccacactc ctccccgaca cccgctgcct ggtggacaac tccaagagtc ggctgcccca
     2281 gctcctggac tgcgacaagg tcaagagcag cctgtacaag cgctggaact tcatccagaa
     2341 tggagccatc atgaacaagg gcacgggacg ctgcctggag gtggagaacc ggggcctggc
     2401 tggcatcgac ctcatcctcc gcagctgcac aggtcagagg tggaccatta agaactccat
     2461 caagtgattg ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2521 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2581 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2641 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2701 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2761 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2821 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     2881 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2941 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3001 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     3061 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3121 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3181 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3241 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3301 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3361 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3421 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3481 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3541 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3601 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3661 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3721 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3781 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3841 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4081 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4561 ggccttttgc tggccttttg ctcacatgtt
//
