LOCUS       sequence-168624-        4815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KIF2B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KIF2B_p.W60L
FEATURES             Location/Qualifiers
     source          1..4815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2694..2793)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2810..2829)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2811..2829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2834..2850)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2834..2850)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3281..4072
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4165..4753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4654..4673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggccagcc agttctgcct ccctgaatcc ccatgtctct cgcccctgaa
      721 acccttgaag ccacatttcg gagacatcca agagggcatc tacgtggcga tccagcgcag
      781 tgacaagcgg atccacctcg ctgtggtcac ggagatcaac agagaaaact attgggtcac
      841 ggtagagttg gtggagaaag cagtcaaaaa aggcaagaag attgacctgg agaccatact
      901 cctgctgaat ccagctctgg actctgctga acaccccatg ccgcccccgc ccttatcccc
      961 cttggctctg gcgccctctt cggccatcag ggaccagcgt accgccacga aatgggttgc
     1021 gatgatcccc cagaaaaacc aaacagcctc aggggacagc ctggatgtga gggtccccag
     1081 caaaccttgt ctgatgaagc agaaaaagtc tccctgcctc tgggaaatcc agaaactgca
     1141 ggagcagcgg gaaaagcgca ggcggctgca gcaggagatc cgagctagac gcgccctcga
     1201 tgtcaatacc agaaacccca actacgaaat catgcacatg atcgaagagt atcgcaggca
     1261 cctggacagc agcaagatct cagtcctgga gcccccgcaa gaacatcgca tctgcgtctg
     1321 cgtgaggaag cggcctctca accagcgaga gacaaccttg aaggacctgg atatcatcac
     1381 cgtcccctcg gacaatgtgg ttatggtgca tgagtccaag caaaaggtgg acctcactcg
     1441 ctacctgcag aaccagacct tctgcttcga ccatgccttc gatgacaaag cctccaacga
     1501 gttggtgtac cagttcaccg cccagccact ggtggagtcc atcttccgca agggcatggc
     1561 cacctgcttt gcctatgggc agacgggaag tgggaagacg tacaccatgg gtggagactt
     1621 ttcaggaacg gcccaagatt gttctaaggg catttatgct ttggtggcac aggatgtctt
     1681 tctcctgctc agaaactcca catatgagaa gctggacctc aaagtctatg ggacattttt
     1741 tgagatttat gggggcaagg tgtatgattt gttgaactgg aagaagaagc tgcaagtcct
     1801 tgaggatggc aatcagcaaa tccaagtggt cgggctgcag gagaaagagg tgtgttgtgt
     1861 ggaggaagtg ctgaacctgg tggaaatagg gaatagctgt cggacttcca ggcaaacatc
     1921 tgtcaacgct cactcatcca ggagccatgc agtgttccag atcatcctga agtcaggagg
     1981 gataatgcat ggcaagtttt ccctcgttga tttagctggg aatgaaagag gagcagatac
     2041 aaccaaggcc agccggaaaa ggcagctgga aggggcagag attaacaaga gtcttctagc
     2101 cctcaaagaa tgtattctgg ctttgggtca gaacaagcct cacaccccat tcagagccag
     2161 caaactcaca ctggtgctcc gggactcctt tataggccag aactcctcca cttgcatgat
     2221 tgctaccatc tctccgggga tgacctcttg tgaaaacact ctcaacactt taagatatgc
     2281 aaacagagta aaaaaattaa atgtagatgt aaggccctac catcgtggcc actatccgat
     2341 tggacatgag gcaccaagga tgttaaaaag tcacatcgga aattcagaaa tgtcccttca
     2401 gagggatgaa tttattaaaa taccttatgt acagagtgag gagcagaaag agattgaaga
     2461 ggttgaaaca ttacccactc tgttagggaa ggataccaca atttcaggga agggatctag
     2521 ccaatggttg gaaaacatcc aggagagagc tggtggagta caccatgata ttgatttttg
     2581 cattgcccgg tctttgtcca ttttggagca gaaaattgat gctctgaccg agatccaaaa
     2641 gaaactgaaa ttattactag ctgacctcca cgtgaagagc aaggtagagt gattgccaac
     2701 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     2761 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     2821 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     2881 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
     2941 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
     3001 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     3061 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     3121 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     3181 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     3241 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc
     3301 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     3361 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     3421 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3481 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     3541 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     3601 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3661 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3721 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3781 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3841 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     3901 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     3961 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     4021 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     4081 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     4141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4801 ttttgctcac atgtt
//
