LOCUS       sequence-168626-        4587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WBSCR17 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WBSCR17_p.A398V
FEATURES             Location/Qualifiers
     source          1..4587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2466..2565)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2582..2601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2583..2601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2606..2622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2606..2622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3053..3844
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3937..4525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4426..4445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggcttcac tgagaagagt caaagtgctg ttggtgttga acttgatcgc
      721 ggtagccggc ttcgtgctct tcctggccaa gtgccggccc atcgcggtgc gcagcggaga
      781 cgccttccac gagatccggc cgcgcgccga ggtggccaac ctcagcgcgc acagcgccag
      841 ccccatccag gatgcggtcc tgaagcgcct gtcgctgctg gaggacatcg tgtaccggca
      901 gctgaatggc ttatccaaat cccttgggct cattgaaggt tatggtgggc ggggtaaagg
      961 gggccttccg gctactcttt ccccggctga agaagaaaag gctaagggac cccatgagaa
     1021 gtatggctac aattcatacc tcagtgaaaa aatttcactg gaccgttcca ttccggatta
     1081 tcgtcccacc aagtgtaagg agctcaagta ctccaaggac ctgccccaga tatccatcat
     1141 attcatcttc gtgaacgagg ccctgtcggt gatcctgcgg tccgtgcaca gtgccgtcaa
     1201 tcacacgccc acacacctgc tgaaggaaat cattctggtg gatgacaaca gcgacgaaga
     1261 ggagctgaag gtccccctag aggagtatgt ccacaaacgc taccccgggc tggtgaaggt
     1321 ggtaagaaat cagaagaggg aaggcctgat ccgcgctcgc attgagggct ggaaggtggc
     1381 taccgggcag gtcactggct tctttgatgc ccacgtggaa ttcaccgctg gctgggctga
     1441 gccggttcta tcccgcatcc aggaaaaccg gaagcgtgtg atcctcccct ccattgacaa
     1501 catcaaacag gacaactttg aggtgcagcg gtacgagaac tcggcccacg ggtacagctg
     1561 ggagctgtgg tgcatgtaca tcagcccccc aaaagactgg tgggacgccg gagacccttc
     1621 tctccccatc aggaccccag ccatgatagg ctgctcgttc gtggtcaaca ggaagttctt
     1681 cggtgaaatt ggtcttctgg atcctggcat ggatgtatac ggaggagaaa atattgaact
     1741 gggaatcaag gtatggctct gtgggggcag catggaggtc cttccttgct cacgggtggc
     1801 ccacattgag cggaagaaga agccatataa tagcaacatt ggcttctaca ccaagaggaa
     1861 tgttcttcgc gttgctgagg tctggatgga cgattacaag tctcatgtgt acatagcgtg
     1921 gaacctgccg ctggagaatc cgggaattga catcggtgat gtctccgaaa gaagagcatt
     1981 aaggaaaagt ttaaagtgta agaatttcca gtggtacctg gaccatgttt acccagaaat
     2041 gagaagatac aataataccg ttgcttacgg ggagcttcgc aacaacaagg caaaagacgt
     2101 ctgcttggac caggggccgc tggagaacca cacagcaata ttgtatccgt gccatggctg
     2161 gggaccacag cttgcccgct acaccaagga aggcttcctg cacttgggtg ccctggggac
     2221 caccacactc ctccccgaca cccgctgcct ggtggacaac tccaagagtc ggctgcccca
     2281 gctcctggac tgcgacaagg tcaagagcag cctgtacaag cgctggaact tcatccagaa
     2341 tggagccatc atgaacaagg gcacgggacg ctgcctggag gtggagaacc ggggcctggc
     2401 tggcatcgac ctcatcctcc gcagctgcac aggtcagagg tggaccatta agaactccat
     2461 caagtaccca actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2521 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     2581 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2641 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac
     2701 cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc
     2761 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     2821 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2881 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     2941 gcctcgggca tccaagcagc aagcgcgtta cgccgtgtgt cgatgtttga tgttatggag
     3001 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga
     3061 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     3121 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     3181 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     3241 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     3301 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     3361 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     3421 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     3481 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     3541 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     3601 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     3661 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     3721 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     3781 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     3841 ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4081 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4561 cttttgctgg ccttttgctc acatgtt
//
