LOCUS       sequence-168628-        4674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SAMHD1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SAMHD1_p.D218H
FEATURES             Location/Qualifiers
     source          1..4674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2553..2652)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2669..2688)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2670..2688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2693..2709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2693..2709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3140..3931
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4024..4612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4513..4532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgcagcgag ccgattccga gcagccctcc aagcgtcccc gttgcgatga
      721 cagcccgaga accccctcaa acaccccttc cgcagaggca gactggtccc cgggcctgga
      781 actccatccc gactacaaga catggggtcc ggagcaggtg tgctccttcc tcaggcgcgg
      841 tggctttgaa gagccggtgc tgctgaagaa catccgagaa aatgaaatca caggcgcatt
      901 actgccttgt cttgatgagt ctcgttttga aaatcttgga gtaagttcct tgggggagag
      961 gaagaagctg cttagttata tccagcgatt ggttcaaatc cacgttgata caatgaaggt
     1021 aattaatgat cctatccatg gccacattga gctccaccct ctcctcgtcc gaatcattga
     1081 tacacctcaa tttcaacgtc ttcgatacat caaacagctg ggaggtggtt actatgtttt
     1141 tccaggagct tcgcacaatc gatttgagca tagtctaggg gtggggtatc tagcaggatg
     1201 tctagttcac gcactgggtg aaaaacaacc agagctgcag ataagtgaac gagatgttct
     1261 ctgtgttcag attgctggac tttgtcatga tctcggtcat gggccatttt ctcacatgtt
     1321 tcatggacga tttattccac ttgctcgccc ggaggtgaaa tggacgcatg aacaaggctc
     1381 agttatgatg tttgagcacc ttattaattc taatggaatt aagcctgtca tggaacaata
     1441 tggtctcatc cctgaagaag atatttgctt tataaaggaa caaattgtag gaccacttga
     1501 atcacctgta gaagattcat tgtggccata taaagggcgt cctgaaaaca aaagcttcct
     1561 ttatgagata gtatctaata aaagaaatgg cattgatgtg gacaaatggg attattttgc
     1621 cagggactgc catcatcttg gaatccaaaa taattttgat tacaagcgct ttattaagtt
     1681 tgcccgtgtc tgtgaagtag acaatgagtt gcgtatttgt gctagagata aggaagttgg
     1741 aaatctgtat gacatgttcc acactcgcaa ctctttacac cgtagagctt atcaacacaa
     1801 agttggcaac attattgata caatgattac agatgctttc ctcaaagcag atgactacat
     1861 agagattaca ggtgctggag aaaaaaagta tcgcatttct acagcaattg acgacatgga
     1921 agcctatact aagctgacag ataacatttt tctggagatt ttatactcta ctgatcccaa
     1981 attgaaagac gcacgagaga ttttaaaaca aattgaatac cgtaatctat tcaagtatgt
     2041 gggtgagacg cagccaacag gacaaataaa gattaaaagg gaggactatg aatctcttcc
     2101 aaaagaggtt gccagtgcta aacccaaagt attgctagac gtgaaactga aggctgaaga
     2161 ttttatagtg gatgttatca acatggatta tggaatgcaa gaaaagaatc caattgatca
     2221 tgttagcttc tattgtaaga ctgcccccaa cagagcaatc aggattacta aaaaccaggt
     2281 ttcacaactt ctgccagaga aatttgtaga gcagctgatt cgagtatatt gtaagaaggt
     2341 ggacagaaag agtttgtatg ccgcaagaca atattttgtt cagtggtgtg cagacagaaa
     2401 tttcaccaag ccgcaggatg gcgatgttat agccccactc ataacacctc aaaaaaagga
     2461 atggaacgac agtacttcag tccaaaatcc aactcgcctc cgagaagcat ccaaaagcag
     2521 agtccagctt tttaaagatg acccaatgtg attgccaact ttcttgtaca aagttggcat
     2581 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2641 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2701 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2761 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     2821 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     2881 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     2941 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3001 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3061 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3121 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3181 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3241 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3301 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3361 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3421 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3481 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3541 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3601 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3661 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3721 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     3781 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     3841 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     3901 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     3961 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
