LOCUS       sequence-168631-        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SYCP1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SYCP1_WT
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3124..3223)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3240..3259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3241..3259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3264..3280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3264..3280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3711..4502
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4595..5183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5084..5103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5337..5354
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5513..5540)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5632..5718)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggaaaag caaaagccct ttgcattgtt cgtaccaccg agatcaagca
      241 gcagtcaggt gtctgcggtg aaacctcaga ccctgggagg cgattccact ttcttcaaga
      301 gtttcaacaa atgtactgaa gatgattttg agtttccatt tgcaaagact aatctctcca
      361 aaaatgggga aaacattgat tcagatcctg ctttacaaaa agttaatttc ttgcccgtgc
      421 ttgagcaggt tggtaattct gactgtcact atcaggaagg actaaaagac tctgatttgg
      481 agaattcaga gggattgagc agagtgtatt caaaactgta taaggaggct gaaaagataa
      541 aaaaatggaa agtaagtaca gaagctgaac tgagacagaa agaaagtaag ttgcaagaaa
      601 acagaaagat aattgaagca cagcgaaaag ccattcagga actgcaattt ggaaatgaaa
      661 aagtaagttt gaaattagaa gaaggaatac aagaaaataa agatttaata aaagagaata
      721 atgccacaag gcatttatgt aatctactca aagaaacctg tgctagatct gcagaaaaga
      781 caaagaaata tgaatatgaa cgggaagaaa ccaggcaagt ttatatggat ctaaataata
      841 acattgagaa aatgataaca gcttttgagg aacttcgtgt gcaagctgag aattccagac
      901 tggaaatgca ttttaagtta aaggaagatt atgaaaaaat ccaacacctt gaacaagaat
      961 acaagaagga aataaatgac aaggaaaagc aggtatcact actattgatc caaatcactg
     1021 agaaagaaaa taaaatgaaa gatttaacat ttctgctaga ggaatccaga gataaagtta
     1081 atcaattaga ggaaaagaca aaattacaga gtgaaaactt aaaacaatca attgagaaac
     1141 agcatcattt gactaaagaa ctagaagata ttaaagtgtc attacaaaga agtgtgagta
     1201 ctcaaaaggc tttagaggaa gatttacaga tagcaacaaa aacaatttgt cagctaactg
     1261 aagaaaaaga aactcaaatg gaagaatcta ataaagctag agctgctcat tcgtttgtgg
     1321 ttactgaatt tgaaactact gtctgcagct tggaagaatt attgagaaca gaacagcaaa
     1381 gattggaaaa aaatgaagat caattgaaaa tacttaccat ggagcttcaa aagaaatcaa
     1441 gtgagctgga agagatgact aagcttacaa ataacaaaga agtagaactt gaagaattga
     1501 aaaaagtctt gggagaaaag gaaacacttt tatatgaaaa taaacaattt gagaagattg
     1561 ctgaagaatt aaaaggaaca gaacaagaac taattggtct tctccaagcc agagagaaag
     1621 aagtacatga tttggaaata cagttaactg ccattaccac aagtgaacag tattattcaa
     1681 aagaggttaa agatctaaaa actgagcttg aaaacgagaa gcttaagaat actgaattaa
     1741 cttcacactg caacaagctt tcactagaaa acaaagagct cacacaggaa acaagtgata
     1801 tgaccctaga actcaagaat cagcaagaag atattaataa taacaaaaag caagaagaaa
     1861 ggatgttgaa acaaatagaa aatcttcaag aaacagaaac ccaattaaga aatgaactag
     1921 aatatgtgag agaagagcta aaacagaaaa gagatgaagt taaatgtaaa ttggacaaga
     1981 gtgaagaaaa ttgtaacaat ttaaggaaac aagttgaaaa taaaaacaag tatattgaag
     2041 aacttcagca ggagaataag gccttgaaaa aaaaaggtac agcagaaagc aagcaactga
     2101 atgtttatga gataaaggtc aataaattag agttagaact agaaagtgcc aaacagaaat
     2161 ttggagaaat cacagacacc tatcagaaag aaattgagga caaaaagata tcagaagaaa
     2221 atcttttgga agaggttgag aaagcaaaag taatagctga tgaagcagta aaattacaga
     2281 aagaaattga taagcgatgt caacataaaa tagctgaaat ggtagcactt atggaaaaac
     2341 ataagcacca atatgataag atcattgaag aaagagactc agaattagga ctttataaga
     2401 gcaaagaaca agaacagtca tcactgagag catctttgga gattgaacta tccaatctca
     2461 aagctgaact tttgtctgtt aagaagcaac ttgaaataga aagagaagag aaggaaaaac
     2521 tcaaaagaga ggcaaaagaa aacacagcta ctcttaaaga aaaaaaagac aagaaaacac
     2581 aaacattttt attggaaaca cctgaaattt attggaaatt ggattctaaa gcagttcctt
     2641 cacaaactgt atctcgaaat ttcacatcag ttgatcatgg catatccaaa gataaaagag
     2701 actatctgtg gacatctgcc aaaaatactt tatctacacc attgccaaag gcatatacag
     2761 tgaagacacc aacaaaacca aaactacagc aaagagaaaa cttgaatata cccattgaag
     2821 aaagtaaaaa aaagagaaaa atggcctttg aatttgatat taattcagat agttcagaaa
     2881 ctactgatct tttgagcatg gtttcagaag aagagacatt gaaaacactg tataggaaca
     2941 ataatccacc agcttctcat ctttgtgtca aaacaccaaa aaaggcccct tcatctctaa
     3001 caacccctgg atctacactg aagtttggag ctataagaaa aatgcgggag gaccgttggg
     3061 ctgtaattgc taaaatggat agaaaaaaaa aactaaaaga agctgaaaag ttatttgttt
     3121 gattgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     3181 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     3241 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3301 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     3361 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     3421 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     3481 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3541 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     3601 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     3661 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     3721 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3781 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3841 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3901 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3961 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     4021 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     4081 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     4141 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     4201 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     4261 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     4321 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     4381 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     4441 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     4501 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     4561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5461 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     5521 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     5581 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     5641 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     5701 agcctttcgt tttatttgat gcct
//
