LOCUS       sequence-168637-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP2K1, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP2K1_p.P124S
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..80
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(239..266)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(358..444)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     493..515
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     507..524
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     508..524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    540..639
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1825..1924)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1941..1960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1942..1960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1965..1981)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1965..1981)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2412..3203
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3296..3884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3785..3804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
       61 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      181 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
      241 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
      301 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
      361 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
      421 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
      481 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
      541 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
      601 gcttttttat aatgccaact ttgtacaaaa aagttggcac catgcccaag aagaagccga
      661 cgcccatcca gctgaacccg gcccccgacg gctctgcagt taacgggacc agctctgcgg
      721 agaccaactt ggaggccttg cagaagaagc tggaggagct agagcttgat gagcagcagc
      781 gaaagcgcct tgaggccttt cttacccaga agcagaaggt gggagaactg aaggatgacg
      841 actttgagaa gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc aaggtctccc
      901 acaagccttc tggcctggtc atggccagaa agctaattca tctggagatc aaacccgcaa
      961 tccggaacca gatcataagg gagctgcagg ttctgcatga gtgcaactct tcgtacatcg
     1021 tgggcttcta tggtgcgttc tacagcgatg gcgagatcag tatctgcatg gagcacatgg
     1081 atggaggttc tctggatcaa gtcctgaaga aagctggaag aattcctgaa caaattttag
     1141 gaaaagttag cattgctgta ataaaaggcc tgacatatct gagggagaag cacaagatca
     1201 tgcacagaga tgtcaagccc tccaacatcc tagtcaactc ccgtggggag atcaagctct
     1261 gtgactttgg ggtcagcggg cagctcatcg actccatggc caactccttc gtgggcacaa
     1321 ggtcctacat gtcgccagaa agactccagg ggactcatta ctctgtgcag tcagacatct
     1381 ggagcatggg actgtctctg gtagagatgg cggttgggag gtatcccatc cctcctccag
     1441 atgccaagga gctggagctg atgtttgggt gccaggtgga aggagatgcg gctgagaccc
     1501 cacccaggcc aaggaccccc gggaggcccc ttagctcata cggaatggac agccgacctc
     1561 ccatggcaaa ttttgagttg ttggattaca tagtcaacga gcctcctcca aaactgccca
     1621 gtggagtgtt cagtctggaa tttcaagatt ttgtgaataa atgcttaata aaaaaccccg
     1681 cagagagagc agatttgaag caactcatgg ttcatgcttt tatcaagaga tctgatgctg
     1741 aggaagtgga ttttgcaggt tggctctgct ccaccatcgg ccttaaccag cccagcacac
     1801 caacccatgc tgctggcgtc taattgccaa ctttcttgta caaagttggc attataagaa
     1861 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1921 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     1981 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2041 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2101 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2161 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2221 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2281 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2341 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2401 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2461 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2521 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2581 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2641 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2701 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2761 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2821 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2881 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2941 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3001 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3061 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3121 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3181 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3241 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3961 cccctgattc tgtgg
//
