LOCUS       sequence-168646-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IDH1, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IDH1_p.E190K
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     763..780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(939..966)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1058..1144)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1193..1215
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1207..1224
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1208..1224
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1240..1339
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2588..2687)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2704..2723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2705..2723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2728..2744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2728..2744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3175..3966
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      721 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      781 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      841 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      901 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
      961 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     1021 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     1081 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     1141 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     1201 cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga
     1261 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
     1321 ttgtacaaaa aagttggcac catgtccaaa aaaatcagtg gcggttctgt ggtagagatg
     1381 caaggagatg aaatgacacg aatcatttgg gaattgatta aagagaaact catttttccc
     1441 tacgtggaat tggatctaca tagctatgat ttaggcatag agaatcgtga tgccaccaac
     1501 gaccaagtca ccaaggatgc tgcagaagct ataaagaagc ataatgttgg cgtcaaatgt
     1561 gccactatca ctcctgatga gaagagggtt gaggagttca agttgaaaca aatgtggaaa
     1621 tcaccaaatg gcaccatacg aaatattctg ggtggcacgg tcttcagaga agccattatc
     1681 tgcaaaaata tcccccggct tgtgagtgga tgggtaaaac ctatcatcat aggtcgtcat
     1741 gcttatgggg atcaatacag agcaactgat tttgttgttc ctgggcctgg aaaagtagag
     1801 ataacctaca caccaagtga cggaacccaa aaggtgacat acctggtaca taactttgaa
     1861 gaaggtggtg gtgttgccat ggggatgtat aatcaagata agtcaattaa agattttgca
     1921 cacagttcct tccaaatggc tctgtctaag ggttggcctt tgtatctgag caccaaaaac
     1981 actattctga agaaatatga tgggcgtttt aaagacatct ttcaggagat atatgacaag
     2041 cagtacaagt cccagtttga agctcaaaag atctggtatg agcataggct catcgacgac
     2101 atggtggccc aagctatgaa atcagaggga ggcttcatct gggcctgtaa aaactatgat
     2161 ggtgacgtgc agtcggactc tgtggcccaa gggtatggct ctctcggcat gatgaccagc
     2221 gtgctggttt gtccagatgg caagacagta gaagcagagg ctgcccacgg gactgtaacc
     2281 cgtcactacc gcatgtacca gaaaggacag gagacgtcca ccaatcccat tgcttccatt
     2341 tttgcctgga ccagagggtt agcccacaga gcaaagcttg ataacaataa agagcttgcc
     2401 ttctttgcaa atgctttgga agaagtctct attgagacaa ttgaggctgg cttcatgacc
     2461 aaggacttgg ctgcttgcat taaaggttta cccaatgtgc aacgttctga ctacttgaat
     2521 acatttgagt tcatggataa acttggagaa aacttgaaga tcaaactagc tcaggccaaa
     2581 ctttaattgc caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2641 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2701 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2761 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     2821 accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca
     2881 ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     2941 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     3001 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     3061 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     3121 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
     3181 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     3241 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     3301 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     3361 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     3421 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     3481 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3541 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3601 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3661 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     3721 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     3781 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     3841 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     3901 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     3961 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     4021 tgagcgtcag accccgta
//
