LOCUS       sequence-168648-       12123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive RFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12123)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 12123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PRm
FEATURES             Location/Qualifiers
     source          1..12123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(820..1408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(900..919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1579..2370,2371..2439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2202..2221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2440..2544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2612..2630
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2668..2690)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2790..2809
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(3346..3376,3377..3578)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3765..3812
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    4060..4107
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6008..6682
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6138..6156)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6188..6208)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6406..6425
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6600..6623
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    7530..7577
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    7825..7872
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(8046..8064)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(12123,1..554,555..585)
                     /label=P element 5' end
ORIGIN
        1 ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc tcaagctctt
       61 attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt acataaatga
      121 ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag ccttctttta
      181 tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg agtcgcctcc
      241 ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcttta aagtgggtat
      301 cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac aattttctct
      361 taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg cagcaaaatt
      421 tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa aaaaattcgt
      481 ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg tatacttcgg
      541 taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatgggcca gacccacgta
      601 gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct tgcccgaact
      661 ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt ccgcccattg
      721 gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca accatttgag
      781 gtatactggc accgagcccg agttcaagaa gaaggcgttt ttccataggc tccgcccccc
      841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     1021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2581 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2641 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2701 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2761 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2821 ccatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2881 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2941 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     3001 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     3061 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     3121 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     3181 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     3241 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3301 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3361 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3421 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3481 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3541 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc aggtcgactc
     3601 tagaggatct ggtctagagc ccgggcgaat tcacgcacta tgccgttctt ctcatgtata
     3661 tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca
     3721 gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga
     3781 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaaaacc aaaagcgctc
     3841 tgaagacgca ctttcaaaaa accaaaaacg caccggactg taacgagcta ctaaaatatt
     3901 gcgaataccg cttccacaaa cgaattcacg cactatgccg ttcttctcat gtatatatat
     3961 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     4021 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     4081 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
     4141 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
     4201 taccgcttcc acaaacgaat tcgccggcgc gccggtaccc cactgtccaa aatcgaaaat
     4261 cgccgaaccg tagtgtgacc gtgcggggct ctgcgaaaat aaactttttt aggtatatgg
     4321 ccacacacgg ggaaagcaca gtggattata tgttttaata ttataatatg caggttttca
     4381 ttacttatcc agatgtaagc ccacttaaag cgatttaaca attatttgcc gaaagagtat
     4441 aaacaaattt cacttaaaaa tggattaaga aaagcttgtg taagattatg cgcagcgttg
     4501 ccagatagct ccatttaaaa cacttcaaaa acaataagtt ttgaaaatat atacataaat
     4561 agcagtcgtt gccgcaacgc tcaacacatc acacttttaa aacacccttt acctacacag
     4621 aattactttt taaatttcca gtcaagctgc gagtttcaaa attatagccg gtagagaaga
     4681 cagtgctatt tcaaaagcaa actaacaagg gtcttaaatt ccaaaacacc aatcctaaca
     4741 agccttggac ttttgtaagt ttagatcaaa ggtggcattg cattcaatgt catggtaaga
     4801 agtaggtcgt ctaggtagaa atcctcattc agccggtcaa gtcagtacga gaaaggtctc
     4861 aatttgaaat tgtcttaaaa atattttatt gttttgtact gtggtgagtt taaacgaaaa
     4921 acacaaaaaa aaagtgatac acagaaatca taaaaaattt taatacaagg tattcgtacg
     4981 tatcaaaaac atttcggcac aatttttttt ctctgtacta aagtgttacg aacactacgg
     5041 tattttttag tgattttcaa cggacaccga aggtatataa acagcgttcg cgaacggtcg
     5101 ccttcaaaac caattgacat ttgcagcagc aagtacaagt agaaagtaaa gcgcaatcag
     5161 cgaaaaattt atacttaatt gttggtgatt aaagtacaat taaaagaaca ttctcgaaag
     5221 tcacaagaaa cgtaagtttt taactcgctg ttaccaatta gtaataagag caacaagacg
     5281 ttgagtaatt tcaagaaaaa ctgaatttca aggtctttgt tcggccattt tttttttatt
     5341 caacgctcta cgtaattaca aaataagaaa ttggcagcca cgcatcttgt tttcccaatg
     5401 aattggcatc aaaacgcaaa caaatctata aataaaactt gcgtgttgat tttcgccaag
     5461 atttattggc aaattgtgaa attcgcagtg acgcatttga aaattcgaga aatcacgaac
     5521 gcactcgagc atttgtgtgc atgttattag ttagttgttt agttaattga agtattttac
     5581 caacgaaatc cacttatttt tagctgaaat agagtaggtt gcttaaacaa agccacgtct
     5641 gaaaatttct tattgcttgt agttgtgacg tcaccatata cacacaaaat aatgtgtatg
     5701 catgcatttc agctgtgtat atatacatgc acacactcgc aacacgaaaa cgatgacgaa
     5761 gcaacggaac aaaggtttct caactaccct ttgttccctg tttcttcgct ttcctttgtt
     5821 ccaatattcg tagagggtta ataggggttt ctcaacaaag ttggcgtcga taaataagtt
     5881 tcccattttt attccccagc caggaagtta gtttcaatag ttttgtaatt tcaacgaaac
     5941 tcatttgatt tcgtactaat tttccacatc tctattttct gcccgcagaa taatccaaag
     6001 cgatcgcatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
     6061 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6121 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     6181 ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga
     6241 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6301 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggctgctt
     6361 catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa
     6421 gaagactatg ggctgggagc cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     6481 gggcgagatc cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     6541 gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggactccaa
     6601 gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcgccga
     6661 gggccgccac cacctgttcc tgtacaagag caggcacaga aggcatcgcc agcgctctag
     6721 gagccgcaat cgcagccgaa gtcgcagcag tgaacgaaaa cgccgtcaac ggagccgaag
     6781 tcgcagcagt gaacgaagac gctacttgta cagcggccgg ccgcgactct aggcgatcgc
     6841 gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggg
     6901 gggatcgatc ccccagatcc gtacgatcct ctagtaaaaa aaaaacaaaa ttttagtgtg
     6961 tataattagg ctaaacaact ggaggattca ttgctccctt tgagtttata ctttttgtaa
     7021 caagaacagg cgcaacactc gggagaattt tgcatttcat ttgtgaataa acgaatacaa
     7081 taaatacaaa tacacgaaaa gaagtcagct gtcgagtttt tgttggtttt tttttccctt
     7141 tttagcttcg aatctgcaaa tgtatttgca tttaagtaaa ccggcttttg ttgggtgtcg
     7201 ttaactttca aggggtctta tgtacttatt ctgttttcct tgtccttgaa ttggacttgt
     7261 ggaaaattaa atggtcagcc aattgcttaa cctttgtcga cctgcggccg cggccggcct
     7321 aggccttaat taagatctga tcatatgttt aaacgtacgc gtgacgtcgg cgcgccattt
     7381 aaatgaattc ctgcaggacg cactatgccg ttcttctcat gtatatatat atacaggcaa
     7441 cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt
     7501 tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta
     7561 gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag acgcactttc
     7621 aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa taccgcttcc
     7681 acaaacgaat tcacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc
     7741 agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga
     7801 agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagaaag
     7861 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa
     7921 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa
     7981 cgaattcgct agcttaatca ctagtgaatt cgcggccgca gatctgttaa cgaattctag
     8041 tatgtatgta agttaataaa accctttttt ggagaatgta gatttaaaaa aacatatttt
     8101 ttttttattt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
     8161 tcgatccaag ggtatttgaa gtaccaggtt ctttcgatta cctctcactc aaaatgacat
     8221 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
     8281 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacctag cgtcaatgtc
     8341 cgccttcagt tgcactttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     8401 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     8461 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     8521 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     8581 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     8641 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     8701 aaaccgcaat cgcacaaaca aatagtgaca cgaaacagat tattctggta gctgtgctcg
     8761 ctatataaga caatttttaa gatcatatca tgatcaagac atctaaaggc attcattttc
     8821 gactacattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     8881 acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta
     8941 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
     9001 acgcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc
     9061 tgccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca
     9121 cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc
     9181 ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca
     9241 ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc
     9301 ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga
     9361 acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc
     9421 ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca
     9481 acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt
     9541 tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc ggatgccacg
     9601 acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct
     9661 cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg
     9721 agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg
     9781 cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa
     9841 gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct
     9901 gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac
     9961 tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct
    10021 agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca
    10081 atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc
    10141 gtgatcggat cgccaagata tggcgacaat tttgctatta gcaaagtagc ccgggatatg
    10201 gagcagttgt tggccaccaa aaatttggag aagccactgg agcagccgga gaatgggtac
    10261 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
    10321 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
    10381 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagtt
    10441 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
    10501 ttgcagaaaa taaataaatt tcatttaact cgcgaacatg ttgaagatat gaatattaat
    10561 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
    10621 gggccaacaa ctcacgcaag tgggcgtgat gaatatcaac ggagccatct tcctcttcct
    10681 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcttg tttagaatac
    10741 atttgcatat taataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
    10801 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
    10861 ttctgggcaa aacgattgcc gaattaccgc tttttctcac agtgccactg gtcttcacgg
    10921 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
    10981 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
    11041 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
    11101 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
    11161 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
    11221 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
    11281 tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
    11341 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
    11401 agtagccgac atatatccga aataactgct tgtttttttt tttaccatta ttaccatcgt
    11461 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
    11521 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
    11581 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
    11641 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
    11701 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
    11761 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
    11821 gcgatttttg tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc
    11881 tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga
    11941 gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg
    12001 catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat
    12061 aataataaga atacatttaa tttagaaaat gcttggattt cactggaact agaattaatt
    12121 cgg
//
